I need method for broth micro dilution assay for AST testing (ISO 20776-1:2019) It would be great if anyone could share a PDF Thanks :) ?
See the attachment. David Booth
I am trying to quantify the release of Nitric oxide to test the Immunogenicity of my antigen on U937 cells (Monocytes). Based on the literature, I am supposed to convert 1x105 cells/ml to...
27 June 2024 3,131 2 View
Dear All, I am currently working on extracting surface water temperature for small lake using Landsat collection 2 level 2 surface temperature product. I have used QA_pixel band to identify water...
26 April 2024 1,750 0 View
Dear all, Can anybody help me to correctly write the Damper Location Matrix for a high rise building where dampers are provided at all floors. Thanks in advance From Bhargav
03 December 2023 2,310 2 View
Dear sirs, I am working on high rise buildings. To determine the response (displacement, velocities, acceleration) State Space Method is used in MATLAB model. Can anybody help me to properly...
01 December 2023 9,719 2 View
Dear all, I am performing Time History(Linear) in ETABS using IS 1893-2016 along with static and Response Spectrum analysis. If the downloaded TH from PEER database is in the unit of G and I am...
14 July 2023 317 1 View
Dear all I have modeled Viscous damper in ETABS using link but when I perform TH analysis and view the display cumulative response, the energy in VD remains zero. I may me making some mistakes in...
14 July 2023 9,499 0 View
Propose an innovative mechanism or device to minimize/avoid the identified risk factor in any working environment. .
19 May 2023 1,217 2 View
Dear respected all I want to work on 76 story benchmark building problem. I want to know still the problem exist or not? Because I have tried to get problem MATLAB code from SSTL 2002 for third...
11 January 2023 1,713 1 View
04 January 2023 1,720 1 View
Dear all I am working on 76 story benchmark building with dampers. can anybody help me to download MATLAB code for 76 story benchmark buildings from SSTL 2000. I have tried 3rd generation code for...
02 January 2023 9,341 0 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
While doing AST for Pseudomonas aeruginosa, after incubation, no zone of inhibition observed in the plate near the well. wells surrounded by bacterial growth, when the same plate observed under UV...
25 July 2024 9,229 1 View
Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....
23 July 2024 7,292 3 View
In 2018 the OHSAS 18001 was improved and progressed into ISO 45001. This was characterized by one or two short falls. I am now reviewing the ISO 45001, with the aim of looking at the entire...
18 July 2024 9,489 0 View
when we grow biofilm in test tube, the pipette often do not reach near the content. so we have to decane it directly or use a smaller test tube.
17 July 2024 3,532 0 View
I'm currently using Roche anti-DIG-AKP Fab fragments to perform Southern Blot assays, but this reagent is delayed since november last year. It's anybody facing the same problem? If so, how are you...
15 July 2024 6,668 0 View
What techniques or assays will be used when a purified lyophilized powder is purchased from a company to study protein-protein interaction?
15 July 2024 1,598 3 View
Requirement for compliance with ISO 1702
13 July 2024 3,918 0 View
Studying a specific monogenetic disease based on mutations in different individual genes. I try to recreate the disease phenotypes of donors in vitro. I knocked out a gene x with above 90%...
12 July 2024 6,313 0 View