Can meta-analysis be done using SPSS?

I have SPSS software and I am unable to find out how to perform Hierarchical summary receiver operating characteristic curve, Deek's Funnel plot and Forest plot. Can please some one guide me how...

25 August 2020 7,469 11 View

How do I make thin film of pristine graphene by spin coating?

I am working with liquid phase exfoliation method and want to cast a film of pristine graphene on glass as well as PET substrate. I have tried to make it with graphene dispersion but it is so...

29 May 2018 5,618 4 View

Does anyone have any experience extracting bacteriophage DNA using the Qiagen Dneasy blood and tissue kit ?

From what I've seen most people use either manual extraction, Norgen Biotek's phage DNA isolation kit, or Promega's Wizard DNA clean up system. I was wondering if the Qiagen Dneasy blood and...

23 May 2018 7,852 6 View

What parameters can be used for processing aeroacoustics in a DES CFD simulation ?

Hi, I am going to simulate a 2D aeroacoustics simulation in Fluent using a DES model on a stator. Actually, the simulation should be performed on a complete 3D rotor-stator setup but due to some...

20 March 2018 7,914 3 View

Is there any technique to differentiate nuclear DNA and mitochondrial DNA?

03 October 2016 6,264 4 View

Does polymer to drug ratio affects the pattern or % drug release from BSA Nanoparticles?

I am preparing Drug loaded BSA Nps So, How 1:300 and 1:5 ratio of drug to polymer affect the % drug release or its pattern?

19 September 2016 9,558 1 View

How can the mass flow rate be parametrized as an input for periodic boundary conditions ?

Hello everyone.I have performed a set of validation simulations using Fluent to compare the numerical results with the experimental results.The steady simulations have been performed on a periodic...

04 August 2016 8,644 5 View

Is there any free online server to carry out molecular dynamic simulation of protein along with ligand(s)?

Usually ligands are not accepted by servers only protein molecule is considered. I would be interested in simulation of protein along with the ligand or ligands.

23 June 2016 1,621 11 View

How do I separate pristine graphene from water/acetone liquid phase solution?

13 June 2016 3,786 3 View

How does electric utilites manage power cut for short duration during real time network reconfiguration application to a distribution network?

07 May 2016 8,614 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

How to simultaneously perform multiple biochemical assays?

I am a research scholar working on heavy metal stress on plants. I will be doing biochemical characterisation( protein, carbohydrate, proline, antioxidant enzymes and many more assays) at interval...

01 March 2021 6,999 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

RIA. How to measure?

When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...

28 February 2021 2,133 3 View

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future ?

I have a time series data of some biochemical studies ( DNA and Chromosomal damage) which I intend to use to further predict into future without necessarily conducting the assay for an extended...

23 February 2021 7,842 1 View

What is the standard protocol in doing replicates for C. elegans lifespan assay on solid media (thru worm picking)?

I would like to know what is the standard protocol in the number of worms per plate and number of plates per concentration of extract X in a lifespan assay done in solid media and not automated...

23 February 2021 9,933 1 View

Why anti-CD20 or other antibodies are not used to label target cells in flow cytometry t-cell cytotoxicity assay?

New to the field of cell therapy. Why fluorescent-labeled antibodies to target cell antigens are not used for determining target cell count by flow cytometry in cytotoxicity assay (eg car-t)? Cell...

21 February 2021 3,278 3 View

Can anyone help or comment on solubilising chlorobutanol in aqueous injections in minimum time possible?

Please suggest solubilisation of chlorobutanol in injections. Tried pH and high temperature. But the issue is of assay drop. If anyone can suggest any solution. Its a commonly used excipient for...

21 February 2021 7,397 1 View

Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

Are exogenous molecules transferred between human cells during doubling ?

Hello RG community, I wonderer whether exogenous molecules (e.g. reporter plasmids, miRNAs or other artificial molecules) are transferred between cultured human cells during their doubling ? So,...

21 February 2021 6,633 3 View