I need help with this liver section - what do you see?

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I need help to identify what it is you can see in this liver section. I have read about granulomas in the fish liver. Might this be one?

03 April 2016 3,798 1 View

Toluidine blue Staining for morphometric analysis of nerve?

To who this may concern , I am trying to stain cross-section of a nerve with toluidine blue for morphometric analysis of the section. I am trying to identify the fibre diameters and general...

02 March 2020 6,297 3 View

TCAD synopsys sentaurus- How to overwrite a default model parameter of mobility models of SDEVICE for a particular program?

Hello!! I am currently facing a problem where I need to specify different mobility values for different regions of my structure but I can't find a way to do it. In manual and existing examples I...

01 February 2020 8,967 4 View

How to prevent getting fragmented villi structure in HE staining of small intestine sections?

I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...

01 February 2020 5,762 3 View

Wanted an integer as input. Found a floating point number as input. 1 4 3.0 26 1.0 ?

I am optimizing a molecule in the ground state, but getting the following error message: Wanted an integer as input. Found a floating point number as input. 1 4 3.0 26 1.0 I am using the...

01 February 2020 516 1 View

Why are there tissues found in my DNA extracts?

I performed DNA extraction using the Quiagen Blood & Tissue Kit on a set of liver samples; and happened to find tissue in the final DNA extract (black precipitate at the bottom). I did add the...

01 February 2020 3,533 3 View

Thickest sections possible for slide-mounted IHC of FFPE mouse brain tissue?

I am currently trying to section FFPE mouse brains using a rotary microtome for fluorescence IHC. I'd prefer to collect the sections free floating in buffer, stain them free floating and then...

01 February 2020 1,993 1 View

Troubleshooting: Alcian Blue staining of chondrogenic differentiated mesenchymal stem cells (3D-micromass cultures), help?

Hello, I'm currently trying to stain 3D-micromass cell cultures obtained from differentiating equine mesenchymal stem cells in chondrogenic induction medium with Alcian Blue 8GX (done in 15 mL...

01 February 2020 6,714 1 View

How would I prevent detachment of tissue section during the process of reticulin staining ?

when I am staining my liver sections with reticulin stain, the tissue lost and detatched from the slide after adding the Ammoniacal silver solution, how can I prevent that?

01 February 2020 9,851 0 View

I would like to use immortalized human adult hepatocytes for my studies.Could anybody advise me which cell line(company, vendors) is better to use?

My project is focused on liver regeneration. I need to choose human adult liver cell lines for my experiments but not quite sure which company is preferable. I need normal (non-cancer)...

01 February 2020 3,585 3 View

What are the best choice of antibodies to distinguish a pro-inflammatory from a pro-repair macrophages population response?

We are trying to evaluate tje host response to implantation of a biomaterial in a tissue defect. So the antibodies should work on paraffin tissue sections

01 February 2020 2,810 1 View