Hi,
There are a number of kits available, such as:
1. QuickExtract™ Bacterial DNA Extraction Kit (by epicentre)
2. ChargeSwitch® gDNA Mini Bacteria Kit (by Life Technologies)
3. QiAmp DNA mini kit (Qiagen).
Hope this helps.
Kindest regards
Veronica
Estimado Ricardo, ya pase tu pregunta a especialistas de mi Centro que trabajan en el Laboratorio de Biología Molecular para darte una respuesta que te satisfaga, un abrazo, Lourdes
You can refer to the following links:-
1. http://www.academicjournals.org/article/article1380298319_Xiao%20et%20al.pdf
2. http://www.dissertationtopic.net/doc/1578372
Dear sir, in our lab we generally practiced extracting DNA from Vibrio bacteria is the "boiling method" the simplest, cheapest & effective method for extraction. Especially when you have lots of Vibrio isolates need to screen them in that case if you use a kit for extraction that will be quite expensive.
For specific molecular work, the CITAB DNA extraction method will be cheap & effective.
"boiling followed by immediate cooling is the easier method for initial screening, After that CTAB method is the one of the effective method for extraction.
If you are going for the identification then go for colony pcr with 16s RNA primer.
Article An Extremely Simple and Effective Colony PCR Procedure for B...
1) Isolate and purify your vibrio culture.
2) Inoculate in any enrich broth (I will use BHI broth) and incubate for overnight.
3) Go for Phenol Chloroform Isoamyl alcohol procedure for DNA extraction. This will give maximum yield but unpurified DNA. Machery-Nagel commercial kit is available for this procedure. You can purchase and go with the kit also or conventional method is also ok.
4) https://www.sciencedirect.com/topics/biochemistry-genetics-and-molecular-biology/phenol-chloroform-extraction