I would like to use dates in statistic methods and I need all files on .raw extension.
I am doing my thesis on the nutrient deprivation on the macroalgae Rhizoclonium sp. I will be analysing the levels over a period of roughly 5 days by HPLC-UV and GC/MS. I will be using seawater as...
28 February 2021 6,477 1 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
Hi, so basically... I am trying to mimic a clinically observed condition wherein a frame-shift mutation happens only at one allele of the patients, giving rise to a truncated variant which evades...
17 February 2021 1,130 6 View
Hello! I would like to know if there is a way to flush/remove all the cells out of a tissue and keep only the extracellular matrix, in order to analyse its composition using e.g. proteomics....
14 February 2021 1,602 4 View
Study bringing together genomics, proteomics etc together
02 February 2021 2,856 2 View
I planned an experimental setup using 10-plex TMT with 9 conditions and 1 bridge channel to compare 5 replicates. But since I experienced some run-to-run variation, I was wondering if a better...
31 January 2021 3,405 3 View
I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.
30 January 2021 3,081 3 View
Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...
25 January 2021 8,333 3 View
We keep getting this error on our Agilent 6470 TQ. We were told it could either be our N2 generator or filter traps. We've called the manufacturer of our generator and they believe it's working...
21 January 2021 2,969 1 View