Dear all,
I would like to ask your advice to measure accurate reaction kinetics of as photochemical reaction using UV-Vis radiation.
My concern is to have a constant an reproducible source of UV-Vis radiation in order to compare the different kinetic studies and also to keep the temperature constant and controlled (I have used a 40W lamp for initial tests to irradiate an NMR tube and I found that the sample was slightly heated up).
I would perform these experiments preparing the reaction mixture in NMR tubes or UV-Vis cuvettes, then irradiate them with the light source and then measure the NMR or UV-Vis at different irradiation times.
There is any commercial device for this kind of experimental set-up?
Many thanks for your advice
Best regards
Hey,
You could use a Stopped Flow device coupled with UV-Vis.The device basically consists of several injection chambers and a mixing chamber which can be exposed to various kind of radiations. The time resolution goes down to the order of miliseconds. This way you can measure in realtime the emission/absorption of either your reactant or the product. The system is easily connected to a cooling or termostated system so you can vary the temeperature as you want.
The device is commercially available, although the price range for a complete setup may go up to 40 000 euros. Our lab utilises the BioLogic setup, you can look up their offers in the link.
All the best,
Istvan
http://www.bio-logic.info/rapid-kinetics-spectroscopy-bio-lab/options/mos-500-accessories/stopped-flow/
For slower reactions a fiber optic UV-vis such as the ones from Ocean Optics can be used to collect UV-vis spectra in real time as you irradiate your sample. Depending on your settings you can collect spectra about once a second.
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