I need a protocol for TBARS assay in human plasma

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Aamal Ghazi Mahdi Al-Saadi

https://www.caymanchem.com/pdfs/700870.pdf

http://www.zeptometrix.com/docs/PI0801192.pdf?1456512819

Sai Prathiba

Can anybody tell me the TBARS assay protocol in human plasma ?

The procedure which I used was 0.5ml plasma plus 1.5ml of 20 percent TCA in HCL followed by centifugation for 3400 RPM for 10 minutes 1ml of the supernatant plus 2 ml of 0.67 percent TBA and the tubes were mixed and kept at 65 degree celsius for 20 minutes but pink colour was not formed .Pls temme the irregularities in this procedure

I Searched online Iam not getting the proper protocol could you please help me in detail.

Is it fine if I will use a water bath at 65 degrees ?

Thanks a lot