Our group is working on genotyping ALDH2 and ALDH3A1. We need a quick and cost effective method. PCR RFLP is a good method or a method with multiplex PCR for both would be even better. Looking forward to hear from the RG community. Kind regards.
what about target-next generation sequencing? fast and cost efficient, but you need a nexseq 500 or ONT platform.
My master's research is in info retrieval and text mining. I would be grateful if you could help me to select a good topic for my phd research proposal.
24 February 2021 393 14 View
I am working on a project monitoring crop field health. For this purpose, I am preparing my dataset using drone video. I have converted it into keyframes. Now I want to pre-process these aerial...
25 December 2020 7,529 5 View
I want to know about the dangling bond? by which method we can find it, does its need some special type of characterization just like XRD etc... or method i want to find dangling bond effect in...
26 October 2020 1,938 6 View
Hi, can someone explain which algorithm should I use to convert DN(Digital Number) values to radiance, because there are many different algorithm that I found, there are 3 for now (based on...
29 August 2020 1,479 2 View
During this pandemic caused by COVID_19 there has been a trend in the academics also due to the modernization by attending online Webinars and even some international conferences are being...
09 August 2020 342 17 View
Can you please erase the doubt from my mind regarding 'Pay and Publish' versus 'Free Publication,' because in both the cases money is being demanded either from the author or the reader. In the...
02 August 2020 1,536 8 View
What are the best tactics, suggestions and recommendations that one should adopt while converting a Ph.D. thesis into a Book and who can be the genuine or reputed publishers for it?
02 August 2020 5,260 14 View
I'm currently doing a study on potential hydrogen damage in superalloys. I'm wondering if carbide dissolution takes place in superalloy in the same manner as steel does with the potential...
12 July 2020 1,420 1 View
Hi everyone I need help in preparation samples for RAMAN spectroscopy, i am working in thin film field, spin coated on ITO or Si wafer. Can any one suggest RAMAN sample preparation method ?...
25 May 2020 7,050 9 View
Dear All, I got 2 weeks old paraffin blocks of tumor masses. I want to extract total RNA from them. The available reagents in my lab are: 1) Cell dissociation buffer (GIBCO#13151-014) 2) TRI...
30 March 2020 5,067 5 View
Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...
02 March 2021 4,029 3 View
I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...
01 March 2021 8,544 1 View
I'm looking to try out an off-the-shelf EEG set for some exploratory work. Curious if anyone has used the OpenBCI, and how much set-up work is required. I've heard they are more difficult to get...
01 March 2021 4,128 3 View
To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...
01 March 2021 8,683 4 View
Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?
01 March 2021 5,311 1 View
I would like to have ideas about which theories of group formation are suitable for entrepreneurial training projects, with the aim of creating bonds, building trust, and collaboration for...
28 February 2021 4,223 2 View
Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.
28 February 2021 5,008 4 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...
26 February 2021 4,777 4 View
Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...
26 February 2021 5,029 2 View