Junbo Yang

5 Questions 28 Answers 0 Followers

Questions related from Junbo Yang

Can you suggest any approaches or tools that I could use to locate conserved regions of multiple large genomes?

I am working on identifying conserved regions in multiple bacterial genomes or larger genomes quickly, but they are too large for multi-alignment. Can you suggest any alternative approaches or...

24 May 2023 8,819 4 View

How to amplify multiple target sites at the same time in a multiplex-PCR system?

I'm trying to amplify 8 targets in 1 tube by multiplex-PCR, but I can't get all the products by next generation sequencing. How to make it?

13 February 2023 3,701 4 View

PCR question. Dose the virus genome different with others?

I'm trying to design a primer for Pestivirus C. I confirmed the potential accessions of this virus in my sample by mNGS and designed 6 universal primer pairs for these acc. These primer pairs...

10 February 2023 4,420 7 View

I want to design a multiplex-PCR based primer set to detect all pandemic pathogens.

Is it feasible to design a primer set for the identification of all the pandemic pathogens (including virulence or drug resistance genes of pathogens)?

01 January 1970 8,963 4 View

How to halt the PCR reaction upon reaching a specified threshold of amplicon copies

In this way, when performing multiplex PCR, it allows all PCR products to reach a consistent level by stopping the reaction when the amplicon numbers reach a specified threshold.

01 January 1970 9,867 4 View