I need a comprehensive list of all lab techniques used within life science. I don't need explanations, just a list of the techniques (E.g western blot etc). Where can i find such one? Thank you in advance,
Best
Simon Molgaard.
Great idea. Let's start creating a list here, i will create the list based on what is written in here, and in case you want it afterwards i will happily share it :)
I will give it a go first, and i hope you will add as many as you can remember. If you could get others to add as well then it would be great!
- Immunohistochemistry
- Immunocytochemistry
- Western blot
-Southern blot
- Northern blot
-qPCR
-rtPCR
-immunoprecipitation
-Isolectric focusing
- Microscopy
-confocal microscopy
-electron microscopy
-immunnoEM
- cell culture of immortalised cells
-cell culture of primary cell
-DNA sequencing
-eastern blot
-far-eastern blotting
-Far-western blotting
-Ligase chain reaction
-northwestern blot
-Plasmid
Cloning of plasmid
-cloning of virus
_reverse northern blot
-Run-off trancription
-Sanger sequencing
-Cell sorting
-Single cell sequencing
Or you could compile them from the methods sections of publications. It's going to be a VERY long list!
Katie and Rahul, thank you for your replies. I am well aware that the list will be extremely long, so i was hoping that somebody had already done it :)
Hi Yuan-Yeu. I am working in neuroscience, but the list should be covering all aspects of life science, not only my own. Do you know of such a list?
Hi Simon Molgaard,
It will be difficult to find a such list, containing all lab techniques from all areas of life science. For example, technique lists for molecular labs usually don't cover the tachniques for plant taxomomy (ex. how to do plant tissue dissection with microtome).......But, I will let you know, if I come across one.
Rahul Roy,
Then, I think, we have to find experts from different life science areas first....
I suggest we try to establish one by each person listing the techniques he can think of in his field.I think it will be a good start.
Great idea. Let's start creating a list here, i will create the list based on what is written in here, and in case you want it afterwards i will happily share it :)
I will give it a go first, and i hope you will add as many as you can remember. If you could get others to add as well then it would be great!
- Immunohistochemistry
- Immunocytochemistry
- Western blot
-Southern blot
- Northern blot
-qPCR
-rtPCR
-immunoprecipitation
-Isolectric focusing
- Microscopy
-confocal microscopy
-electron microscopy
-immunnoEM
- cell culture of immortalised cells
-cell culture of primary cell
-DNA sequencing
-eastern blot
-far-eastern blotting
-Far-western blotting
-Ligase chain reaction
-northwestern blot
-Plasmid
Cloning of plasmid
-cloning of virus
_reverse northern blot
-Run-off trancription
-Sanger sequencing
-Cell sorting
-Single cell sequencing
Size exclusion chromatography
ion exchange chromatography
agarose gel electrophoresis
high performance liquid chromatography
bradford method of protein quantification
here I'm writing some of my lab techniques, please find those if these are useful to you.
Plasmid isolation
Digestion with Restriction enzymes
DIALYSIS (Electroporation)
Agarose gel electrophoresis
Gram staining
MALDI Tof
mass spectroscopy
flow cyto-metry
Serial dilution
enumeration of bacteria from soil, water, sewage samples by isoaltion, enumeration methods by using microbial techniques.
plating methods- Spread plate, pour plate, streak plate methods
WBC, RBC, Platelets counting
Haemoglobin estimation by Shali's method
IMVIC tests
Thin layer chromatography
paper chromatography
absorption chromatography
Blood groups detection method by using antiseras
Widal test
Dear all. Thank you very much for all the help, it is much appreciated!
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