I don't have any melt curves in realtime PCR with e-cadherin primers. what is your idea?
Lack of melt curve might be due to the lack of product in your reactions. You should first load your qPCR products on the agarose gel to see if you can detect any product. If there is not any product, that's obvious enough that your PCR conditions are not suitable and you should check everything from primers to the concentration of all PCR components. In this situation, I recommend optimizing the reactions with PCR machine followed by agarose gel electrophoresis, instead of qPCR so that you can save more money.
But, If you can detect a band, it means that you should change the setting of your device to be able to detect your products during melt-curve processing. You should encompass 50 to 95-degree temperature in the setting, which guarantees the detection of any band.
Simplest answer is that you did not get amplification. Run out the samples on an agarose gel and look for bands.
Another possibility is you didn't add enough fluorescent dye (or the dye is old) or the detection is set to the wrong wavelength. Did you see amplification of your products in the realtime machine? Or did you do standard PCR and just looking at the meltcurve?
Finally, make sure you include a robust positive control and a negative control. That will help you troubleshoot any problems.
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