Which is the best solvent/ solution for the storing intestinal biopsy sample at -80 deg Celsius? and for how long can I store it ?

I need the tissues for the isolation of leukocytes for drug metabolite analysis. How long will they be stable at - 80 deg Celsius.

30 September 2016 3,751 1 View

I need a protocol for the isolation of leukocytes from intestinal mucosal tissue and which solvent to be used to store the tissues before isolation?

19 September 2016 8,924 3 View

Which is an appropriate column to be used for the analysis of nucleotides like thioguanine mono/di/tri phosphates for LC-MS ?

If possible kindly provide me a reference for the same.

19 September 2016 1,633 4 View

Validation of overexpression by microinjection, any ideas?

Hello everyone, I am overexpressing a gene in Zebrafishes, by using some neural crest cell promoter, through microinjection. I do see some phenotype, but so far, I have seen it in a transgenic...

14 March 2016 2,396 8 View

How can one include voltage limits as a multiobjective for power flow problem using Genetic Algorithm?

27 October 2014 1,787 3 View

Can anyone help with molecular dynamics -PCA analysis in gromacs?

I have performed molecular dynamics simulation for 50ns in Gromacs. The protein is stabilized after 20ns. I did PCA analysis for last 30,20,10 and 5 ns. Is there any criteria to select the time...

05 June 2014 6,583 2 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Why are the velocities so high in my Boussinesq approximation Fluent simulation?

Hello, I have a mechanism where air rises due to convective flows. In this simulation, nothing is driving the air except this. I understand that the Boussinesq approximation only works for small...

02 March 2021 4,910 3 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View