I have ordered a standard custom oligo and intend to do Cy3 tagging. However, do I need to modify the oligo first so the tagging is possible?

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Andrew Reed

Yes. A lot of fluorescent dye labeling relies on simple reactions such as Click chemistry or maleimide chemistry for protein labeling. For DNA, our lab uses an nhs ester amine reaction to site specifically label a DNA substrate containing a nucleotide base modified with an additional amine functional group. You can order DNA with these modifications directly from IDT and get free dye from various sources.

We describe it in this paper: http://pubs.acs.org/doi/abs/10.1021/acs.biochem.6b00166

Fina Nhm

Thank you for the feedback! So, with the standard oligo I have in hand i.e. no modifications, it is possible to add an amine group via ester amine rxn? By doing so, I could proceed with the Cy3 tagging, yes?