Andrew Reed

2 Questions 8 Answers 0 Followers

Questions related from Andrew Reed

What is a possible explanation for multiple primer insertions during site-directed mutagenesis?

I am using the QuikChange XL kit to make point mutations into a pET30a construct. I've had this occur with several primers sets but also had those same primers work other times. I have tried...

13 May 2015 2,224 3 View

Does anyone have an explanation for observing multiple primer insertions during site-directed mutagenesis?

I am using the QuikChange XL kit and I have designed the primers using the tool provided by Agilent and calculated the Tm using their equation. Also, I used IDTs oligo analyzer to check for...

12 May 2015 9,527 1 View