Fina Nhm

3 Questions 4 Answers 0 Followers

Questions related from Fina Nhm

Is there a difference between carrying out resuspension using Tris-HCl and TE Buffer?

I am working on the resuspension of a fluorescent tagged oligonucelotide. I came across few protocols carrying out the process with either Tris-HCl or TE Buffer. The only difference in composition...

19 October 2017 4,746 6 View

Would curcumin create a noise and produce false positive results in the experiment involving with Cy3 dye?

I am intending to use curcumin and Cy3 dye together on microarray. I am aware that curcumin itself is fluorescent. But, I am more focused on Cy3 dye excitation to read the relative fluorescent...

27 September 2017 3,344 1 View

I have ordered a standard custom oligo and intend to do Cy3 tagging. However, do I need to modify the oligo first so the tagging is possible?

I ordered the standard oligo with no modifications. I am aware in order to tag with Cy3, there needs to be an amine group. Is there in such a way I can modify the oligo so that the Cy3 tagging is...

29 August 2017 9,166 2 View