Hello, researchers! I'm working with polystyrene nanoplastics (100 nm) in in vitro studies/cell culture. I have a lot of doubts about the preparation of the working solution. Firstly, my question is: is it better to use cell culture medium or ultra-pure water to dilute the nanoplastics? And secondly, is it better to use a vortex or a sonicator to mix the solution? Thank you in advance!
When preparing a working solution for polystyrene nanoplastics (100 nm) in in vitro studies, it's better to dilute the nanoplastics in **cell culture medium** rather than ultra-pure water to maintain particle stability in the same environment as the cells. For mixing, using a **sonicator** is recommended over a vortex, as it ensures better dispersion and prevents nanoparticle aggregation. Monitoring particle stability over time with techniques like dynamic light scattering (DLS) is also advised.
Hello! Thank you for your response, Raghad Mouhamad. First, I used ultra-pure water to prepare the stock solution of nanoplastics. Should I use cell culture medium to prepare the working solution with the concentration range I need, or should I continue using ultra-pure water?
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