Hi! I want to combine LPS injection with social defeat stress protocol to do research in inflammation. For technical reasons, I have to choose a dose that generates as less discomfort as possible. Does someone know the effect of LPS injection for rats in terms of discomfort? I was checking some publications in which they used from 0.1 to 2.5 mg/kg. Which dose in between can be one which does not gnerate rat discomfort more than level 2? (according to EU laws?
In general, it has been proven that stress could induce chronic sub-clinical inflammation. Also, repeated LPS exposure has been used previously to study depression. Having said that, it would be better to conduct a pilot study for different LPS doses. I think this review article may have the accurate answer for your question.
Article Stress Induces Endotoxemia and Low-Grade Inflammation by Inc...
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