I am trying to isolate DNA from Orchid plant containing high polysaccharide.
Would also suggest idea protocols.
Hello Yash,
First of all i would suggest you to measure the purity of DNA and then go for measuring the concentration of the DNA, Below are the few links you could follow
1. https://www.promega.in/resources/pubhub/enotes/how-do-i-determine-the-concentration-yield-and-purity-of-a-dna-sample/
2. http://www.nanodrop.com/Library/T009-NanoDrop%201000-&-NanoDrop%208000-Nucleic-Acid-Purity-Ratios.pdf
and try to follow the other method for DNA isolation if possible,
1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC230171/pdf/353311.pdf
2. https://www.google.co.in/url?sa=t&rct=j&q=&esrc=s&source=web&cd=9&cad=rja&uact=8&ved=0ahUKEwip2vyet7_QAhWKRo8KHW14CcgQFghSMAg&url=https%3A%2F%2Fwww.researchgate.net%2Ffile.PostFileLoader.html%3Fid%3D56cee1d564e9b228358b4574%26assetKey%3DAS%253A332974873694218%25401456398804997&usg=AFQjCNFmXQTSwX-haWXYf7KFS3Z9bcrqLA&sig2=hwncQ9w7vM87g3_7ezC1Vw&bvm=bv.139782543,d.c2I
3. https://www.le.ac.uk/biology/phh4/dnaiso.htm
4. https://www.ncbi.nlm.nih.gov/pubmed/8593035
Hope these few links will solve your problem
https://www.researchgate.net/post/How_to_quantify_DNA_using_OD
For now, Due to some technical problems in lab i am just performing gel electrophoresis.
I will try to follow other provided protocols.
Thank you so much for your time and support!
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