Absorbance (A) = (Aλ vis-max x A700) pH 1.0 0 (Aλ vis-max x A700) pH 4.5
TAC (mg/L) = (A × MW × DF × 1000)/(ε × l)
MW: the molecular weight, calculated as cyanidin-3-glucoside (449.2); DF: the dilution factor;
l: the cuvette radius, 1 cm; ε: the molar absorptivity, calculated as cyanidin-3-glucoside (26,900).