Hi, I'm evaluating the biological activity of a plant extract and in the HPLC analysis we found a compound with a peculiar UV spectrum. I still need to do chromatography tests to purify the compound and do an NMR, but does anyone have any idea what compound it could be? It has a peak at 222 and a double peak at 280.
The UV spectrum with peaks at 222 nm and a double peak at 280 nm suggests the compound could be a phenolic compound, such as a flavonoid. Further purification and analysis using NMR and MS are necessary to confirm its identity.
A UV-Vis absorption peak at 222 nm is commonly associated with π-π transitions* in compounds with conjugated systems, including: Benzene and its derivatives (phenol, aniline, toluene) Polycyclic aromatic hydrocarbons (PAHs), heterocyclic copmpounds like Nucleic acid bases (purines: adenine, guanine; pyrimidines: cytosine, thymine)
- Heteroaromatic rings (indoles, pyrroles, quinolines), there could also be conjugated cmpounds like α,β-Unsaturated carbonyl compounds (e.g., chalcones, enones)
- Conjugated polyenes (e.g., β-carotene derivatives)
- at peak 280nm , it ignifies presence of protein , neuclotides or aromatic ketones
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