Abdullah Tahir It looks really degraded and has RNA contamination. Please, re-isolate if you need to do any experiments with it. The genomic DNA will be not far from the wells, when compared with your DNA ladder this is definitely not intact genomic DNA.
Abdullah Tahir
Yes, you can. The bright wide bands are just RNA, which can be removed by treating them with RNase. The gDNA is not plenty, but it is plenty enough for PCR.
There is high molecular weight dna which will pcr amplify well and the pcr product will digest fine. If you meant that you also wanted to restriction cut the original dna for a southern blot then you will have to remove the large amount of rna first then measure the amount of dna to cut because the smaller rna will look like dna to a spectrometer and then you will cut too little dna to get good results
If you just want to do PCR on it, yes. I've found PCR will work even with quite degraded DNA or DNA with lots of impurities. If you want to sequence this DNA, you might have issues.
This is degraded, inconsistent yield, when your PCR fails, you will be left with more questions then answers. Reisolate.
many good answers... it may help to add that if you want to improve the consistency of your PCR results (and it will be impacted by variable high conc. of RNA) you may want to treat with RNase, then precipitate, dry and then resuspend in 10mM Tris-HCL pH 7.8-8.0 (or, use Qiagen columns from a kit for isolating genomic DNA). The re-precipitation part was missing from otherwise good answers. Good luck
This is not that pure DNA isolation as it supposed to be, however, you can still use it for PCR. The reason is that it looks like this isolate is a bit degraded.
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