I have been trying to troubleshoot my DNA extraction process using Plant DNA kit (I won't mention the brand). However, I've been getting the same results. My extracted genomic DNA remains on the well. I used 1% AGE and also 0.8%. I also used proteinase K as included in the kit. What could be the problem? I tried to amplify the genomic DNA, but it was not successful. I used Actin as control, but it was not successful as well.

Notes:

* I used Ethidium bromide.

* I used 6x loading dye

* I used freshly prepared TAE pH 8.0

* I followed the exact protocol as written in the manual. I even did some troubleshoots, like using refrigerated centrifuge, increasing/decreasing incubation time, addition of leaf samples from 30mg (as written in the manual) to 100mg.

* I make sure that my DNA template is not contaminated. I always maintain it in ice all throughout the procedure, unless otherwise stated in the manual.

* I am using Lambda DNA/Hind III marker.

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