Hello Abdmajid,

There are quite a few commercially available NO donors that work well in cell culture. They differ primarily by NO-relasing time (e.g. fast vs slow releasers). We had good experience with DETA NONOate (20 μM) (Crit Care Expl 2020; 2:e0194

DOI: 10.1097/CCE.0000000000000194), Spermine-NONOate (500 μM)(doi:10.1016/j.mvr.2007.02.008), and SNAP (S-nitroso-N-acetylpenicillamine, 1000 μM) (doi:10.1016/j.mvr.2008.06.001).

Hope that will help you to narrow down the NO-donor of your choice.

Best regards