Is it possible that the phosphorylated proteins mobilize faster than the non-phosphorylated ones?

The band downshifts on Western-blot, after kinase assay treatment.

23 December 2020 7,959 3 View

Species identification.Who can tell me the scientific names of these Gastropods?

Species identification. I've been doing research on communities of marine macrobenthos recently, but some of them can't be identified, which hinders my research. Who can help me identify these...

14 December 2020 8,357 3 View

TOPO cloning fails to integrate my PCR product?

Hi everyone. I am currently using Invitrogen pCR-Blunt II-TOPO kit to ligate my PCR fragments into the TOPO vector. However, most of the time I obtained very few E.coli colonies (fewer than 3 on...

09 July 2020 1,314 3 View

White stuff found on the surface of insect cell supernatant?

Hi everyone. Just out of curiosity. I am using High Five insect cell as the protein expression system. Cells are resuspended in 50mM Tris-HCl (pH 8.4), 200mM NaCl, 5% glycerol. After cell...

09 July 2020 6,764 6 View

Could anyone help me to determine the structure of this molecule?

Dear all, I need to determine the structure of the following molecule, see the addached file, could anyone help me? Thanks very much!!!

22 June 2020 9,128 1 View

Thermal sprayed zirconia protective coating on ceramic setter plate for MLCC sintering?

Dear all, Hope you and your families are healthy and well-prepared in the current COVID-19 situation. Could you please shed some light on the mechanism of using zirconia protective coating...

19 March 2020 1,893 4 View

MATLAB how convert 1 column text image to 2D image?

I got a output image in text file after done a simulation which is 1 column vector(1x290400). Can somebody teach me how can I convert this text file to an exactly image same as below. Thank you...

10 August 2019 9,188 7 View

Cohesive element in Abaqus is created by python?

hello,all ! recently i try to use cohesive element to analyse interface damage .but i have trouble in creating cohesive element with python.hope that all give me some recommendation such as...

06 June 2019 3,826 3 View

No title

01 June 2019 5,308 3 View

Any dispersant that can stabilize alumina powder in water at pH>=13?

Dear friends, I am trying to disperse alumina particles at pH>=13 in water, which is tough. I know this situation is not common. pH

19 August 2018 3,446 2 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Expression cloning by using cDNA,do I need to modify the cDNA first?

I am going to have a expression cloning of mammalian gene by using shuttle plasmid to transforming the E.coli However I don't know I should only inserting the Coding sequence ,or I can...

28 February 2021 5,440 3 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View

PUC19 as a transfer plasmid for lentiviral delivery?

Hello, Is it possible to use pUC19 as a transfer vector to be packed in using the second generation viral particles packaging system( pMD2.G; psPAX2 plasmids)? As far as I understand it there is...

28 February 2021 4,868 2 View

Did anyone ever try using pLVX-TetOne Plasmid with a copGFP as a selection marker instead of Puromycin?

When using a lentiviral vector for inducible expression of genes using the Tet-On system. In the literature, I saw a widely used of pLVX-TetOne-Puro Plasmid, is there a reason why people prefer to...

25 February 2021 1,011 3 View

Why do the PCR bands from the cDNA templates (extraction from the cell after transfection) show different size from the band of the plasmid template?

After transfection with the plasmid ( linearized ) and subcloning of the cell lines, RNA was extracted from the cell and then reverse transcripted to cDNA. When PCR reactions were run to verify...

25 February 2021 5,712 3 View

Does anyone have a sequence for the human CD34 promoter?

I'm looking for the human CD34 promoter sequence to engineer into a vector construct. Can anyone recommend a good source for this sequence? Thanks very much, Laura

25 February 2021 8,205 1 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View