I am looking for thesis written in the area of young people and drug usage in Ireland?

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Gromacs first step of minimization ?

I have face this problem anyone help me how to solve this issue ?which is below Fatal error: There are inconsistent shifts over periodic boundaries in a molecule type consisting of 78 atoms. The...

07 August 2024 2,598 1 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

Conjugation of PEG-Amine to an Amino Acid Using EDC?

I am attempting to conjugate PEG to an amino acid at the C-terminus, for the purposes of producing nanoparticles. I have been told that PEG modified with amine groups can be used for this purpose,...

31 July 2024 2,033 1 View

Please inform me about the International Conference on Plant Biology?

Please inform me about the International Conference on Plant Molecular Biology, or Plant Biology, scheduled for December 2024, January/February, or March 2025.

14 July 2024 3,120 7 View

Is there any standard format or protocol for plant photo-plate creation?

need to know protocol for photoplate creation, styles, etc

11 July 2024 8,321 2 View

Unification of General Relativity and Quantum Mechanics is possible ?

How can anyone develop a theoretical framework that successfully unifies general relativity and quantum mechanics? how this new theory should accurately describe gravitational interactions at...

10 July 2024 5,040 8 View

Radial Basis Functions Based Differential Quadrature Method for One Dimensional Heat Equation "required matlab code" and provide code in discription?

I am able to submit the code documents. Although my graph is 1, I would like to be in the 6E-05 graph range. Please, someone correct the error.

08 July 2024 5,770 0 View

My cells need to be treated 4 times (0-72hrs) and then take conditioned media for LC-MS. Can I keep them in serum free for so long? Recommendations?

I have tried to optimize the serum free dmem which is the best but i am afraid the secretory profile has too much variations.

03 July 2024 4,044 3 View

How can we describe the cognitive mechanisms of trauma-processing in simple terms?

Please help me with one or more of the following, if possible: How does a client mentally process the trauma? What are the cognitive domains of processing? What measures are available to assess...

02 July 2024 6,263 0 View

Does anybody know if amino acids like GABA, BABA, proline, lysine, valine or any other non-aromatic amino acid are destroyed by UV light?

I am trying to eliminate infection from my plates and therefore, gave UV treatment after addition of amino acid (used for better growth). Can you please suggest if UV destroys amino acids or not,...

02 July 2024 2,922 1 View

Is changing the medium during the experiment overestimate the results?

I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...

07 August 2024 2,283 2 View

What condition of Kirat Rai's participation in politics before and now in Nepal?

The political participation of the Kirat Rai community in Nepal has evolved significantly over time: Historical Context Historically, the Kirat Rai people, like many indigenous groups in Nepal,...

05 August 2024 5,950 1 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

Can i quantify a nanoparticle encapsulated in cell culture medium by hplc?

Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...

03 August 2024 2,730 1 View

How to maintain the drug concentration in cell culture study for long term?

I am working in the filed of long acting drugs with Nano formulation. I want to study the efficacy of my formulation in 2D cell cultures while this required media change on every two days. In such...

24 July 2024 8,696 0 View

How can I determine if a drug binds to a ribosome using a Cryo-EM map?

Hello, I am searching for the binding position of a drug in a ribosome, based on previous work indicating it binds there. The resolution of the ribosome-drug Cryo-EM map is around 2.5 Å. I've been...

19 July 2024 2,155 0 View

How to have sustainable development for the artic region?

How can we have both environmental and indigenous people protection together with shipping all year-round, as well as natural resources exploitation?

18 July 2024 1,077 3 View

Are there local (indigenous, native, first nation, aboriginal) definitions of climate anxiety (that do not include western definitions)?

We are writing an essay on climate change and its impact on indigenous people circumpolar north people, native nations, islandic, aborigenal of pacific and so on

16 July 2024 5,248 3 View

How to calculate percentage cumulative drug release?

How to calculate percentage cumulative drug release when, Total volume of dissolution media = 250ml Added formulation was equivalent to 10mg of drug Sample withdrawn = 1ml each time Sink condition...

15 July 2024 4,192 1 View

MTT assay - 6 well plate?

Hi, In the 6 well plate, when I am seeding the cells. Do I add 1ml of complete media to one of the wells for the control at this stage or do I wait unit I am treating the plates to do that? Also...

13 July 2024 9,783 1 View