How to convince the public to wear masks/gloves if they are resistant?

As you all know, this pandemic has taken a major toll on the entire global community. In some societies, people do abide by COVID protective measures. Yet, in other societies people are resistant...

30 September 2020 546 13 View

I am having a problem ligating my vector with PCR product by using the pcDNA3.1 V5-His-TOPO expression kit.Have anybody had such an experience?

I am trying to clone using the pcDNA3.1 V5-His-TOPO expression kit. There is no problem transferring it to the cell, but I can only transfer the vector. I did not succeed in vector-insert...

06 September 2020 3,483 0 View

What are these additional bands in my agarose gel results?

I have included pictures to illustrate my point. In the gel image that has only 4 lanes: Lane 1 is a 1 kb molecular weight ladder. Lane 3 is my wild type DNA (in a pGEX plasmid) and Lane 4 is the...

28 August 2020 4,146 4 View

Delta Furin SARS-COV-2 mutant Western Blot Analysis showed double band around 250 kDa. Processed and unprocessed version? Need evidence, please help?

I analysed SARS-CoV-2 Spike Protein delta Furin mutant in my bachelors thesis and need evidence (paper, research or something like that) to proof my suggestion. I think the double band at around...

26 August 2020 1,058 4 View

How to get SD from CI??

I apologize in advance for my rookie question. I have been looking in the internet how to get SD from CI values, and some sources tell me to apply the formula =SQRT(N)*(CI_upper.limit -...

19 August 2020 8,946 2 View

Who I should contact to get permission to use the BREQ-2 tool in academic research?

The BREQ-2 tool is commonly used in physical activity research to measure motivation. The tool was developed by David A. Markland. I have not been able to find how to ask consent for its use in...

15 April 2020 5,428 3 View

How to aggregate data from various surveys and reflect them onto one person?

So for example, we have the following polls: First poll: People who said they like orange juice for breakfast are as follows: Texas (50%), NY (25%), North Carolina (20%), and Florida (5%) Second...

01 March 2020 2,916 3 View

How do voter data companies collect or match info?

I have been recently studying proprietary voter files and data. While I know that voter files are for the most part public (paid or free), I am confused as to how companies match this data to...

30 January 2020 7,520 1 View

Is Bowtie2 assembly file saving problem caused by no space left on the disk?

Hello, Here is what I got with Bowtie2 while changing .sam file to .bam file: "samtools view -bS egpart1.sam > egpart1.bam [E::bgzf_flush] File write failed (wrong size) samtools view: writing to...

28 December 2019 3,244 2 View

How to lysis an expressing a membrane protein using a plasmid?

I have created a plasmid for my protein of interested tagged with Flag tag. The sequence looks fine but for the life of me I cant get this protein to express itself and see it using western blot (...

30 June 2019 7,043 15 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

How to calculate BREQ 3 questionnaire?

how to calculate BEHAVIOURAL REGULATION IN EXERCISE QUESTIONNAIRE BREQ 3 ,which contain 24 questions and each question has 5 answers (0- Strongly disagree , 1-disagree, 2- Neither agree nor...

02 March 2021 811 3 View

Please suggest a good text book for "Research Methodology in Life Sciences". Which book would be good for PhD Scholars?

Please provide Book Title and author name

02 March 2021 9,059 3 View

How does Scientist/RG community evaluates a case study article and the impact of it on a researcher career?

An article that is published as a case study in the SJR listed journal. How much value is added to a researcher career? How it is evaluated by a scientific researcher/University admission...

02 March 2021 9,661 3 View

Could any one guide me, to write research proposal for grant? ?

We have one plant, the local people using this for controlling blood sugar, the toxicity and antidiabetic activity of the plant is not reported yet, please guide from whag should be the process ?...

02 March 2021 2,300 1 View