MSC isolated from MCP-1 knockout mouse?
Will my PCR product be viable for Sanger Sequencing? I (a dumb undergrad) left my PCR product in the fridge because I was planning on finishing preparing the samples that week to send out but then...
29 February 2024 1,788 2 View
I have been running a series of ITC to look at the binding affinity of Fluoxetine Hydrochloride (Prozac) (0.5mM) with Human Serum Albumin (0.02mM). Prozac is my ligand and HSA is in my sample...
28 February 2024 7,632 3 View
Applications are due November 2,...
30 October 2023 1,460 1 View
Can anyone suggest the source for ZPE scaling factors for Minessota Local Functionals 15 with basis sets lanl2dz and 6-31+g(d,p). Thank you
21 April 2023 8,493 5 View
I am staining cardiac tissue sections which have been fixed in 4% PFA using Phalloidin. When imaging these tissues under fluorescence, there is not a defined f-actin organization or striated...
19 March 2023 9,136 0 View
Kindly share
12 March 2023 4,632 10 View
Implementing a project and hope to enhance collaboration to resolve defects. Which BIM tool will be your preference and why?
12 March 2023 3,814 1 View
We have a protocol that has our mice running 3 hr/day. However, our Exer treadmill has some features that do not promote the encouragement of their running. In pic #1, you can clearly see a...
18 January 2023 9,185 2 View
I'm thinking about 'inverse vulcanized polysulfides' that theoretically are polymer chains of sulfur that are crosslinked with a divinylic monomer (two different sulfur chains can connect to a...
25 June 2022 9,224 2 View
I'm trying to generate a kill curve in MKN-45 cells. I'm going to generate a stable KO in this line. I've found the cells detach at low concentrations of blasticidin-s. 2 ug/mL. I know that MKN-45...
27 September 2021 6,020 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View