Protein Quantification Outside Standard Curve?

I am running a Bradford protein assay and my protein values are falling beyond the standard curve. I am already using 1uL of sample in the standard microplate assay. Is there a way to extend the...

13 December 2020 6,672 3 View

How to analyze sodium lauryl sulfate using HPLC-RID?

Hi, I am analyzing the content of anionic surfactant: sodium lauryl sulfate (SLS) in commercial detergents using HPLC-RID/refractive index detector. I used column Agilent Zorbax Eclipse XDB C-18...

15 November 2020 1,405 2 View

Cs-Gluconate for intracellular solution?

Dear patch-clamp enthusiasts, For patch-clamp recordings of vomeronasal sensory neurons, I need a cesium-based intracellular solution. Because I also need a defined amount of chloride, I have to...

11 November 2020 6,384 7 View

PLS- Is it normal that the standardized regression coefficients of my model do not coincide with the VIP values?

I am trying to perform a partial least square model (PLS) using one response variable and 33 explanatory.. Before the analysis I have standardized all my data so i am expecting that the regression...

19 October 2020 6,702 2 View

Which is preferable for whole rat brain clearing: tert-butanol or xylene?

I am performing LM on whole rat brains with a specific focus on the hippocampus and cannot find which information on which clearing technique is better.

05 October 2020 1,306 1 View

Is recoding negative questions always necessary when downloading to SPSS from SurveyMonkey?

I formatted a survey in SurveyMonkey using questions from a valid and reliable instrument (Principles of Adult Learning, Conti, 1978). Questions used a 6 point Likert scale (Always, Almost...

29 September 2020 8,194 4 View

Clarification on Shapiro-wilk sig. , skewness and kurtosis?

Hello, Maybe is a bit silly question but, I have a variable (N=78) and on shapiro-wilk test the sig. is .008 which is just over (greater) than 0.05 meaning that is just about normally...

29 August 2020 1,384 4 View

How to use Clamping and interpret its outcomes in MAXENT?

For my Masters project I have been creating SDMs using MAXENT, and the next stage involves using future climate projections in the 'projection layers directory' to see potential future...

25 June 2020 2,658 6 View

Google Earth Engine for Multitemporal analysis (Land Use Land Cover Change)?

Hello. I am reading about Google Earth Engine (GEE) and their applications for vegetation, landcover and agriculture mapping. I am analyzing an area of 20Mha to determine a multitemporal changes,...

01 May 2020 2,059 11 View

Why do we use qPCR with a singular reference primer?

Hi, I am doing a qPCR and using a reference primer for my lab research project. However, I am not sure what is the purpose of having a singular primer for my reference gene and having both...

28 February 2020 1,636 3 View

Which is a lucid standard comprehensive book to guide on psychological scale construction and its standardization?

02 March 2021 7,082 3 View

Looking for genomic DNA extraction kits from mouse tissue (centrifuge-based, not spin-column)?

Hello. I am looking to extract genomic DNA from mouse brain and lungs and I need a kit that is not spin-column based because I need to scale up the solution amounts for 400mg tissue (most cap at...

25 February 2021 155 2 View

What are some alternatives to the Self-Directed Learning Readiness Scale (SDLRS) developed by Dr. Lucy L. Guglielmino?

The Self-Directed Learning Readiness Scale (SDLRS), also known as the Learning Preference Assessment (LPA), was developed by Dr. Lucy M. Guglielmino in 1977 to measure learners' attitudes of and...

15 February 2021 3,095 4 View

How do I understand the relationship of age and the answers on Likert scale?

Hi people, I want to see the relationship between 4 age categories and Likert scale questions on SPSS, which method or test is the best to find that out? To be more exact, I want to measure if the...

10 February 2021 2,241 3 View

Where can I find the Affiliate Stigma Scale developed by Mak and Cheung?

Need the scale for a dissertation. Would be better if I could get it for free since my work is not funded. I need the English version of this scale.

09 February 2021 4,022 3 View

Any recommendations for open-source survey form builder, which has option for semantic differential scale questions apart from Likert scale?

Asking this as Google forms doesn't allow multiple items in a semantic differential format.

04 February 2021 5,869 4 View

Can I add a neutral option in a 4-point Likert scale?

Hello, I want to use a motivation scale with my students (secondary school) for my research, but the scale is in a 4-point Likert style with no neutral option (Certainly true - True - Not True -...

04 February 2021 4,757 13 View

How do I scale up a column for preparative isolation/purification from analytical conditions in HPLC?

What protocol should I follow to determine optimum separation conditions using an analytical column in an HPLC system?

03 February 2021 7,288 3 View

Hello friends, happy evening !! I am just wondering if you would know where I can find the (ALTS) altruism scale by S.N.Rai and S Singh to download ?

ALTS is an altruism scale developed by S.N Rai and S.Singh.

02 February 2021 7,243 2 View

How to calculate response rate if the number of eligible respondents is not available?

Hi All, I am carrying out an international research with clinicians (speech-language pathologists/ therapists). International professional associations will be distributing the questionnaire link...

02 February 2021 5,306 3 View