I am having trouble working with G418 selection in Pichia pastoris, does any one have experience with this?

27 April 2021 1 243 Report

I have carried out concentration curves between 0,5 ug/ml and 2 ug/ml in order to determine the Minimal Inhibitory Concentration with no luck. I am working with recombinant strains. Any help is most welcome, many thanks!

Celina Galles

Thanks for your kind answer Stefan Zimmermann

! I will be checking out the paper you reccomend, but it clearly looks like we should try much higher concentrations...

Best regards!

Celina

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