I was able to deglycosylate the denatured version of our protein of interest, but saw no deglycosylation when I tried with the native protein. Any tips?
I was reading about metabolic disorders caused by the lack of production of a protein. Can a possible treatment be to supplement the patient with that lacking protein, or would the body degrade it...
19 November 2020 595 2 View
I am attempting to simulate a triaxial test in ABAQUS/Standard using the clay plasticity model in the ABAQUS material database. I am using a 3-D cylindrical specimen which contains a single...
03 October 2014 6,873 3 View
I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...
27 February 2021 547 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View
Hi, I am a third year chemistry undergrad doing my group project in which I need to predict the HPLC retention time of 20 aromatic molecules. to do this we require the chemical properties of these...
24 February 2021 1,384 3 View
I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...
20 February 2021 199 2 View
Taking a fractional ODE problem as an example. It has come to my attention that if the fractional order parameter is in the interval (0,1], many only presents results for alpha=0.9, 0.99. I saw...
08 February 2021 9,671 3 View
when i saw my DNA target on the electrogram there was a dimer primer below on DNA target. how do i remove it ? what should i do ? thanks
03 February 2021 5,290 3 View
I am trying to increase the yield for the reaction below. I did the column and I saw that the major side-product is the symmetric dimer formed by 4-bromobenzyl bromide. I imagine that NaH is...
20 January 2021 7,874 4 View
Yesterday, I ran a gradient endpoint PCR with some primers and saw positive amplification for positive and negative for negative amplification. Running the same assay today, show positive...
13 January 2021 5,201 1 View
Hi guys: I have been doing CRISPR knock-in and knock-out and one seemingly impossible scenario keeps happening. In the CRISPR knock-in attempt I tried to add a tag on a gene of interest, so I...
07 January 2021 190 3 View
I did SPI and SPEI work through R studio. As you know, r program for each month; We draw results for 1, 3, 6, 9, 12 and 24 months. However, when interpreting the results, we interpret the 3-month...
01 January 2021 8,223 5 View