Can you please recommend a protocol for native protein deglycosylation using PNGase F?

11 December 2019 0 3K Report

I was able to deglycosylate the denatured version of our protein of interest, but saw no deglycosylation when I tried with the native protein. Any tips?

Badges
Science topic

More Celina Galles's questions See All

Why do my cells turn gelatinous upon addition of lysis buffer?

Hello, I work with HaCaT cells, and until now there hasn’t been any issues. But recently I’ve had a problem when lysing the cells. Upon the addition of lysis buffer, the cell “suspension” turns...

28 November 2023 5,248 4 View

Sac-B selection in E coli troubleshooting?

Hello, I need help improving my counterselection procedure when using sac-B. I have grown my E coli strain of interest in LB-agar media with and without sucrose (5%), at 30°C, following the...

08 November 2023 2,292 3 View

Is there a way to estimate doses for systemic/oral administration based on topical concentrations?

Hello, I am setting up a mouse study to test the effects of a number of different compounds in our mouse model. We currently have data on effective concentrations given through topical...

24 May 2021 1,706 3 View

Can someone help me get this paper?

I am doing a school project and I would really like to have this article includedReview of Microfluidic Approaches for Fabricating Intelligen... The issue is my university does not have a...

18 May 2021 3,977 5 View

I am having trouble working with G418 selection in Pichia pastoris, does any one have experience with this?

I have carried out concentration curves between 0,5 ug/ml and 2 ug/ml in order to determine the Minimal Inhibitory Concentration with no luck. I am working with recombinant strains. Any help is...

26 April 2021 235 1 View

In theory, can metabolic diseases, characterized by the lack of production of a protein, be treated (not cured) by supplementing with that protein?

I was reading about metabolic disorders caused by the lack of production of a protein. Can a possible treatment be to supplement the patient with that lacking protein, or would the body degrade it...

19 November 2020 902 2 View

Can anyone help with a load-controlled triaxial test using clay plasticity material model?

I am attempting to simulate a triaxial test in ABAQUS/Standard using the clay plasticity model in the ABAQUS material database. I am using a 3-D cylindrical specimen which contains a single...

03 October 2014 6,998 3 View

What role do research and statistics play in Organizational Psychology?

I am examining the field of Organizational psychology and the difference between practicing OP's and research OP's.

01 February 2013 6,645 3 View

How to extract RNA from neurons?

Hi, I am a master's student who studies inflammation. I'm planning to extract RNA from the Vagal nerve or Dorsal Ganglion roots, but I got a question. It would be only a few 50ug per each nerve,...

23 July 2024 6,783 1 View

What are those black/hollow structures?

Hello, I recently stained my cells with an Arf6 antibody. The healthy control looks pretty normal, while the disease group cells have abnormally large black structures in the cell body. Has anyone...

21 July 2024 1,044 1 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

In sds page and western blot, non specific expressed protein with very thin band is also there along with thick band of specific protein. why ?

Asper discussion, First time my clonning was failed becuase the first 45 nucleotide was not cloned in pet30 a vector then I choosed the different clone of 1:1 ration fraction, I got the size of...

13 June 2024 7,564 7 View

How can I select the narrow band filter in Zetasizer nano ZS?

I am working with chitosan/dextran sulphate nanoparticles loaded with rifampicin, which is fluorescent. Dsl analysis are bad and I saw that is necessary use a narrow band filter. How can I select...

05 June 2024 1,324 3 View

Does anyone know where this module is in Simulink?

I try to build a three-phase wireless power transmission model and saw this module in an article.

27 May 2024 3,471 2 View

How to use dynamic phasor model in Digsilent?

I saw RMS and EMT simulation respectively in Digsilent, but my research focus is on dynamic phasor, so how i can do it? My model is a DFIG connect to IEEE39 system

15 May 2024 6,406 0 View

Why Interior point optimization is suitable for large nonlinear dataset?

I saw interior point optimization technique is performing much faster than other standard non linear optimization techniques for large dataset. I would like to know the reason for this

10 May 2024 2,111 1 View

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week. How to fix?

Today I found a very strange thing in my research gate account..it showing Citations -0..However, I saw more than 150 Citations last week..Dose anyone know how to fix it ??

19 April 2024 1,111 2 View

Do you want people to die in earthquakes?

Do you want people to die in earthquakes? You saw: two days before the earthquake in Taiwan, I had a reliable precursor - a KaY-wave anomaly. Today I checked for an anomaly in China. It was...

03 April 2024 8,674 3 View