Celina Galles

3 Questions 3 Answers 0 Followers

Questions related from Celina Galles

Sac-B selection in E coli troubleshooting?

Hello, I need help improving my counterselection procedure when using sac-B. I have grown my E coli strain of interest in LB-agar media with and without sucrose (5%), at 30°C, following the...

09 November 2023 2,174 3 View

I am having trouble working with G418 selection in Pichia pastoris, does any one have experience with this?

I have carried out concentration curves between 0,5 ug/ml and 2 ug/ml in order to determine the Minimal Inhibitory Concentration with no luck. I am working with recombinant strains. Any help is...

27 April 2021 223 1 View

Can you please recommend a protocol for native protein deglycosylation using PNGase F?

I was able to deglycosylate the denatured version of our protein of interest, but saw no deglycosylation when I tried with the native protein. Any tips?

11 December 2019 3,186 0 View