I am growing E.coli cells in sodium oleate with brij-58 as a surfactant to make it soluble in water. How to solve foaming problem?

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Omar Gonzalez-Ortega

You can use silicone oil.

Alaa AbdulAziz Abed

Silicone oil is good choice

Jinan Sadeq

Hi , Dr Alaa this link may help you

https://www.sigmaaldrich.com/Sigma/Life-Science

Hassan Hammadi

yes, you can use silicone oil.

Shikha Jindal

Thankyou all but can I use vegetable oil or mineral oil?

Can you precisely tell me which silicone-based antifoam and of which brand?

Sandeep Rellegadla

Yes, silicone oil is a better choice.

Try out this particular product for better results:

http://www.sigmaaldrich.com/catalog/product/aldrich/85390?lang=en®ion=IN

Do not use vegetable oil as the cells could use it as carbon source

Thankyou all...so out of silicone oil and antifoam A which is better?