Shikha Jindal

5 Questions 18 Answers 0 Followers

Questions related from Shikha Jindal

How to convert the biomass calculated through the experiments into carbon % fluxes?

Suppose I have Biomass as follows: 1. DNA - 3.4g/g % 2. RNA - 19.7 g/g % 3. Protein - 44.4 g/g% 4. Carbohydrates - 9 g/g% 5. lipids - 2 g/g% How to convert each entity into carbon atom % ?

06 June 2019 9,921 2 View

Which is the best method among the HPLC, NMR and Enzymatic kit to calculate the uptake rate of acetate?

I have a bacterial culture in which acetate is present.I want to know the uptake rate of acetate from the media. I did NMR but didn`t get much satisfactory results. Should I go for the HPLC or...

03 March 2019 4,702 3 View

I got a lot of peaks when I analyzed FAME through GC-MS.How to identify our peak?

How to identify your actual peak?I am confused by looking at so many peaks that which is the my peak which I am looking forward to it. I used HP-5 column injection temp-50`C Split ratio-...

12 December 2017 1,952 7 View

What are the external metabolites while degrading fatty acids as a substrate? How to quantify oleate in HP-5ms column for GC-MS?

I want to make a standard plot for the oleate by using different concentrations without using derivitization. I want to calculate oleate `s uptake rate in e.coli . Derivitization would be tough...

12 December 2017 8,212 15 View

I am growing E.coli cells in sodium oleate with brij-58 as a surfactant to make it soluble in water. How to solve foaming problem?

INSTRUMENT : Sartorius Biostat Aplus Fermenter As soon as I add the SODIUM OLEATE + BRIZ-58 into the M9 media, it starts huge bubbling. Which antifoam has to be used in this case where sodium...

04 April 2017 5,276 9 View