28 July 2023 1 580 Report

I wanna extract MPs from water samples without harming the attached bacteria. Samples contain not only MPs but also other particles such as organic debris.

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Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,311 5 View

When sequencing a plasmid to confirm transformation has been successful, would I have to sequence the full plasmid?

I have transformed some DH5alpha cells with point mutated proteins placed in pcDNA plasmid and extracted the plasmid; now I have to send the plasmid for sequencing for confirming the presence of...

19 January 2021 5,842 3 View

In ARDL model, coefficient of independent variable not showing significance. Can it happen? How should i read my results?

Hi, i am working on macro economic data in a bi-variate framework with GDP as dependent variable and inflation as independent variable. I have applied bound test plus ARDL model and it confirms...

13 April 2020 3,375 4 View

Any idea about peak identification of non-expandable minerals in EG treated oriented mounts?

During XRD analyses of oriented clay mounts, a number of peaks which are NOT typical of expandable clay minerals have been observed on XRD patterns of EG solvated ones. Ethylene Gycol was provided...

08 January 2019 6,770 1 View

Why there are two protein chains in Glucocorticoid receptor - DNA binding domain?

Hi, I'm doing a MD study about DBD of Glucocorticoid receptor. Why do it have two Zn motifs ( protein chains)? Both have same function or different functions? What is the importance of having two...

29 August 2017 2,548 3 View

How to do a Molecular Dynamics simulation for DNA molecule without constraint it?

how to do a MD simulation for DNA molecule using AMBER force field in GROMACS? And how to make an one itp file for two complementary strands of DNA?

16 August 2017 7,180 5 View

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What are the most reliable approaches to extract key continuous frames from a video, with complete automation?

I have an input video, of skin (from a microscope that means it is magnified such that blood capillaries are visible). The idea is to extract 15 sec of continuous frames from a video of 1 min,...

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Would fecal samples stored at -20C for over a year impact metagenomic analysis?

Dear All, I have access to some rodent fecal samples that have been stored at -20C instead of -80C. Anyone have experience with the impact of stowage condition on comparative metagenomic analysis...

01 March 2021 2,512 1 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to perform statistical analysis of coefficients extracted from transform techniques?

I am studying the potential of DCT and FFT coefficients extracted from a signal to classify the different objects that emit the signal. I applied the Shapiro-Wilk to confirm that data was not...

01 March 2021 8,079 2 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

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How to remove fatty material from plant extract?

The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.

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