How do a splice correction of a spectrum in ASCII format?

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Panel data analysis using EVIEWS related questions?

Hi, I am running panel data analysis using Eviews. My dataset consists of one dependent variable and 11 independent variables for six countries over 21 years. After conducting multicollinearity...

28 January 2024 3,290 1 View

Getting no DNA bands when running a gel after purification of PCR Products. What may be the reasons?

I have extracted bacterial genomic DNA from CTAB method and done an agarose gel electrophoresis to check the quality. Then I got clear DNA bands (image). PCR was done using 16s rDNA primers....

15 December 2023 6,115 4 View

Near Singular error during Panle ARDL?

I'm currently working with a panel data set that has 6 cross-sections and 19 time periods. In the process, I've encountered issues related to endogeneity, as well as challenges with both...

27 November 2023 8,003 4 View

I am gonna extract attached microorganisms from microplastics. Reliable method?

I wanna extract MPs from water samples without harming the attached bacteria. Samples contain not only MPs but also other particles such as organic debris.

27 July 2023 756 1 View

What do the ghost peaks in this RP-HPLC correspond to?

Hello, I have performed NaCl extraction of a nuclear protein and run it on RP-HPLC with a mobile phase of acetonitrile in 0.1% TFA in water. I had repeatedly observed two tall peaks around minute...

13 April 2022 1,805 0 View

What causes the horizontal smear on neighboring wells of this western?

Hello, I am encountering a strange horizontal smear of neighboring wells of my recent westerns. Now protein quantification is problematic. I am unsure whether this is due to some horizontal...

05 October 2021 1,077 4 View

Measurement scales for Microcredit?

I want to measure the impact of microcredit on women empowerment Can you explain some likert scale questionnaire that I can use to analyse the microcredit

16 September 2021 7,180 1 View

What could bind to ubiquitin monoclonal antibody in nuclei from brain tissues and HEK cells that has MW of ~ 37kDa?

Hello, I have purchased ubiquitin monoclonal antibody Ubi 1: Ubiquitin Antibody (13-1600) Thermofisher; I intended to check the quality of this monoclonal antibody and I run some brain nuclei at...

23 April 2021 2,431 0 View

Has anyone tried growing C2C12 cells in 1X DMEM without L-glutamine?

Hello, I plan to transfect C2C12 cells and I am happy if their growth rate would slow down if they are not provided with glutamine (as they grow so fast)! Has anyone tried growing C2C12 cells in...

05 March 2021 613 3 View

Why has my protein transfer using fresh transfer buffer is worse when compared to protein transfer using old buffer?

Hello, I have performed two westerns in parallel, one using fresh buffer (made with old 10X tris-glycine) and the SDS gel was made 4 days prior to western; and the other western was performed...

03 February 2021 9,557 5 View

What is the prepared reference material that can be used in inductively coupled plasma optical emission spectrometry (ICP-OES) to measure REE and U ?

What is the prepared reference material that can be used in the ICPE-9820 Shimadzu Japan instrument, which employs inductively coupled plasma optical emission spectrometry (ICP-OES) to measure...

06 August 2024 1,896 1 View

Could dyes amplify the spectrum of light to a specific wavelength?

I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...

05 August 2024 3,290 1 View

Is artifacts in XPS possible to build high deviation in binding energy larger than 5 eV??

Hello. Thanks for your consideration to see my question. Recently, I conducted XPS anaylsis of g-CN that is prepared from thermal polycondensation of DCDA, so-called conventional bulk-g-CN,...

30 July 2024 9,824 2 View

Tips for teaching Orton Gillingham+ to students with autism?

I am beginning to use OG+ in my district and noticed during training there are a lot of materials and routines, which may be overwhelming for my students, especially those on the autism spectrum....

30 July 2024 5,695 0 View

There is possible way to calculate experimental DOS from the UPS spectrum of the material. How to calculate the DOS from UPS?

Here, I have attached the UPS graph. I'm trying to calculate the DOS/DOVS from the UPS.

29 July 2024 4,971 1 View

Mass spectra averaging algorithm?

I am now developing a python module for ms2 database searching, would like to realize a function that similar to what Xcalibur did, choose multiple mass spectra and get an averaged spectra. But...

22 July 2024 3,975 1 View

What does an additional peak in FTIR between 3100 and 3300 nm affect the quality of the material, benzethonium chloride USP??

I do not know much about FTIR! The material Benzethonium Chloride USP was compared to a reference standard in regards to FTIR, as per the USP testing: Identification B. Infrared absorption (FTIR),...

16 July 2024 6,918 7 View

What should be the best Lumens range for T8 (120cm) full spectrum LED lamp tubes?

Please (for Arabidopsis), what could be a good Lumens and color range (Kelvin) range for full spectrum LED lamp tubes size T8 (120cm) for each shelve measuring 130x50 cm (length x width) and 60 cm...

11 July 2024 6,078 1 View

MS/MS spectrum with 5 Da difference in m/z value?

Hi everyone, We experienced a strange pattern in some spectra coming from HRMS/MS analysis. In particular, in the MS1 spectrum a base peak at m/z=M and a smaller peak at m/z=M+5Da appear, but in...

08 July 2024 3,631 4 View

For 3d transition metal elements, is there a mathematical expression for the energy E in their hard X-ray absorption spectrum?

Only the absorption side is considered, and the fine structure of the extended side is not considered

05 July 2024 2,894 2 View