I tried this in two organic solvents
1) Heptane
2) Carbontetrachloride
I was performing estimation of Total Soluble Sugar content in bacterial cultures by Anthrone method. Out of 9 samples, only one sample showed positive reading while others had a negative reading...
22 July 2024 9,576 4 View
I was reading papers on plant secondary metabolites and their applications and many of the papers/books I read, have the point that plant-based medicines which are the result of application of...
08 July 2024 9,935 10 View
(Transfection with two plasmids has worked for this cell line. pRL-TK(Renilla) is not getting transfected).
16 June 2024 6,687 2 View
Generally, the chemical substitution should adjust the Fermi level, and the computed TSS indeed suggests that the Fermi pocket for the three samples different, so why are the frequencies extracted...
17 May 2024 1,897 1 View
The sample nature is thin film
17 May 2024 8,459 0 View
I want to define T shape gate I added material air in free space , But it is showing boundry condition not defined electrode num=3 name=gate mat=gold x.min=2.6 x.max=3.5 y.min=0.03...
13 May 2024 3,059 0 View
Generally for bacterial DNA isolation we use a fresh culture of 2-5 days old. But if we use an older culture say 10-15 days old will it have any impact on the DNA content and the DNA isolation...
13 May 2024 3,061 11 View
I want to define T shape gate I added material air in free space , But it is showing boundry condition not defined electrode num=3 name=gate mat=gold x.min=2.6 x.max=3.5 y.min=0...
13 May 2024 5,026 0 View
it would be helpful if you provide the reference to your answer
11 May 2024 5,989 4 View
I'm isolating bacterial from soil on Nutrient Agar media but after 4-5 days I see fungal growth start in the plates. I read about use of Nystatin to prevent fungal growth. How much quantity or...
21 April 2024 6,039 5 View
I have virus (viral hemorrhagic septicemia virus) in suspension and the experiment will not involve cells. What level of TCID50 is preferred?
11 August 2024 3,115 1 View
I got these smeared bands quite often lately. We typically run the gel at 140V with a 10-12% gel and do a wet transfer at 220 mA for 1.5 hr in cold room. We also noticed some dirty spots/dots (see...
10 August 2024 7,480 3 View
I'm currently working on calculating the collision cross section (CCS) for various ions, and I'm facing challenges when dealing with sodiated and multiply charged ions. Most of the resources I’ve...
08 August 2024 8,329 0 View
If we map as a continuous motion an ionising electron (beginning its journey at n=1) in an H atom, a specific hyperbolic spiral appears (see animation). When we solve this spiral formula, we find...
07 August 2024 5,343 2 View
Hello dear colleagues, We have prepared a manuscript on NiTi-based alloys and are seeking a second opinion on our current TEM results. If you are a Ph.D. holder with experience in TEM and have...
07 August 2024 9,563 0 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...
05 August 2024 9,798 3 View
average particle size calculation from TEM
04 August 2024 2,921 1 View