I am doing xanthine oxidase inhibitory potential tests with some plant extracts. Why am I not getting any colour, even in control?

10 October 2013 1 1K Report

I am following protocols using spectrophotometer.

You do not get a colour since the conversion of xanthine to uric acid can be followed using UV/VIS - try phosphate buffer pH 7.4 and measure at 298 nm - check p. 68 from http://ufdcimages.uflib.ufl.edu/UF/E0/02/17/81/00001/affum_a.pdf

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