I am following protocols using spectrophotometer.
You do not get a colour since the conversion of xanthine to uric acid can be followed using UV/VIS - try phosphate buffer pH 7.4 and measure at 298 nm - check p. 68 from http://ufdcimages.uflib.ufl.edu/UF/E0/02/17/81/00001/affum_a.pdf
I am doing an experiment for checking the expression of Hemoxygenase-1 in J774 macrophages after treatment with a compound by western blot. But in untreated control, there is considerable...
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Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !
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I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.
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Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...
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The resultant solid was partitioned three times with 250 ml of petroleum ether (40-60/c) to remove any fatty material and evaporated to dryness.
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