We are collecting sludge from municipal waste, and my aim is to extract the reducing sugars from sludge for further processing. I tried with it, but without success. Is there any simple protocol to extract the sugars.
Thank you in advance.
Hi Kongkona Saikia,
Just one question: the biosolids/sludge were in contact with active microorganisms before you have tried to separate reducing sugars? If yes, you will not find any reducing sugars. Indeed, reducing sugars are the most attractive source of carbon and energy for almost all kind of living microorganisms.
Hi Mariano Gutiérrez-Rojas
Thank you for your answer. We treated the sludge soon after we collected it, which should inactivate the microbes to some extent. We did the reducing sugar estimation after the treatment of the sludge .
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