Given that the concept of operation is based on the liner curve -
y=ax + b
The machine provides me with the sample ABS, as well as the blank ABS;
Sample weight is 1 g.
Extract was made up to 50 ml with distilled water
Good morning,
to get the concentration of your sample solution, you should take your sample absorption ( = y) and then calculate the cation concentration of your sample solution (= x). The correct formula is x = (y-b)/a. Then you should calculate the ammount of cations which is dissolved in 50 ml by multiplication with the correct factor . This result is the ammount of your cation (for which you have done the calibration) in 1 g of your sample.
Best regards,
Timm Wolff
After the AAS measurement you will get the concentration of the prepared sample in ppm (mg/L). multiply the result by the sample solution in L (50/1000 in your case) then multiply by 1000 to covert to microgram then divide on the solid sample weight (1g) to get the concentration of the cation in µg/g which is mg/Kg or ppm. This is the correct factor mentioned by Timm Wolf (50 in your case)
Hi Samuel,
You can try to use my formula in calculating the concentration of metal in a sample.
The formula is : C = x . V . f/W
Where, C : concentration of metal in sample (mg/kg)
x : concentration of metal obtained from AAS (mg/L), x is obtained from the equation of y = ax + b
V : initial volume of the sample solution (L), in your work : 50 mL or 0.05 L
f : dissolution factor (exp. 1 time, 2 times, 3 times, etc)
W : mass of sample (kg)
You can apply this formula to any calculations
Regards
Hi Samuel,
From a linear curve, you will get the concentration of cation in the sample solution in mg/L (ppm). You can find out the concentration of cation in the plant tissue in mg/kg (ppm) using the following formula:
M = C. V / W
Where, M = Concentration of cation in the plant tissue in mg/kg (ppm)
C = Concentration of cation in the sample solution in mg/L (ppm)
V = Made up volume of sample extract in mL
W = Weight of plant tissue taken for extraction in g
If you dilute the sample solution before AAS measurement, multiply the result with dilution factor (e.g., if you dilute 1ml sample solution to 10ml, then multiply the result with the factor 10.
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