How to express accuracy of the neural network where the dataset has not been split into two, training set and test set?

In use, the weights obtained are considered with the greatest accuracy when the data set is divided, because the weights are initialized at random and the training data is swapped at random...

19 January 2021 5,714 4 View

The loss of hyperbolicity in PDE?

Question about the loss of hyperbolicity in nonlinear PDE: when complex eigenvalues appear, what is the effect on flow? I understand that we do not have general results on existence in this case,...

16 December 2020 2,571 3 View

Books on doing a PhD?

Hi colleagues, I wondered if any of you could recommend a book for a student starting out on her PhD. Her subject area is: health, education, training and child...

07 December 2020 549 4 View

Why do I receive the sae mesage several times?

26 October 2020 409 1 View

Is there a method to completely remove OCT from frozen tissue?

I am trying to remove OCT from frozen tissue for DNA/RNA sequencing. My goal is to remove a portion of tissue from the OCT embedded tissue, without thawing or greatly disturbing the remaining...

24 June 2020 1,715 1 View

How to escape local minimum in the training of Convolutional Neural Network?

I am trying to train CIFAR10 dataset on CIFAR10 architecture on MATCONVNET without gpu but my objective function is not decreasing down to 1,665. What may be parameters to tune while considering...

16 June 2020 4,700 8 View

Can distributed intelligence substitute for a lack of ethics?

In the trust economy, ethics becomes a scare resource. To what extend will technology be able to step in and fill this gap? Distributed Cognition and Distributed Morality: Agency, Arti...

03 January 2020 9,711 3 View

What type of water should I use in viral DNA extractions?

I am attempting to set up a QC laboratory to test viral samples isolated from insects. The test will include gel electrophoresis and qPCR analysis. What I want to know is what type of water should...

16 December 2019 683 5 View

Where are the good exposures at the Ditrau Massive in Transylvania, Romania? (Could you give a global grid reference?)?

I am a third year geology student at the University of Edinburgh, School of GeoSciences. This summer I will be working on my dissertation mapping on the Ditrau massive. I have a mapping partner. I...

26 March 2018 9,838 3 View

Unequal error variances in two-way ANOVA?

Using SPSS, N=297. The Levene's statistic is very significant at p

16 August 2017 6,365 3 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Dapi fluorescence protocol for colon cell lines ?

28 February 2021 2,534 2 View

Does RNAiMAX transfection reagent work in serum free medium?

I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...

26 February 2021 10,041 3 View

Has anyone had luck using cell lines other than HEK 293 (such as COS1) to package lentivirus?

I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...

25 February 2021 3,030 1 View

Unknown debris sheets observed in cell culture flasks?

Hi, We culture neural progenitor cells in T175 culture flasks coated with PLO-Laminin. We have successfully grown these cells with our protocol for months. Recently, we observed large sheets...

25 February 2021 3,443 4 View

How do I transfect a large plasmid into PC12 cells with nerve growth factor (NGF) receptor?

I have a large 16 kb plasmid, which I need to transfect into PC12 cells. Lipofectamin 2000 didn't work and with GFP alone the transfection rate is very low. I also tried the neon invitrogen...

25 February 2021 6,635 5 View

How to model Supercritical water gasification (SCWG) process in Homer software?

Hi everyone How to model Supercritical water gasification (SCWG) process in Homer software?

25 February 2021 4,075 1 View