Does HT-29 cells grow in McCoy's 5a Medium Modified (ATCC recommended medium) form tight junctions in normal well plates (not Transwell plates)?
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Hi, everyone. I have some issues with THP-1 cell culture. I have little experience in cell culture and I hope some of you could share some experiences in culturing this type of cell. I'm...
30 May 2024 7,764 1 View
I`m in project about miRNA in function, mutations, process and other for explain in a class. And I`m a autism, I have difficult to understand if the peoples the class understanding my class. So, I...
01 May 2024 2,611 0 View
How could I have the structure of microRNA-183 in 3D to visualize its structures?
25 April 2024 9,113 0 View
Hello, I'm seeking recommendations for safe and reliable software to anonymize CT scan images for research. While DICOMCleaner has been our go-to for removing DOB, names, and other PHI, it's...
03 April 2024 3,717 3 View
This would be an excellent alternative to reducing oral antibiotics for these chronic conditions.
01 January 2024 4,597 0 View
Does anyone works or has worked with immunohistochemistry on tick tissues, who can provide me with a protocol or bibliographic source where I can find a detailed protocol? The papers that use...
11 September 2023 7,764 0 View
Hi everyone, I'm looking for a flow cytometry antibody that recognizes the peptide antigen (preferably OVA 323-339 or a Mtb antigen - but open to other suggestions ) bound to MHCII on mouse...
22 August 2023 795 0 View
It is about internal medicine. If so, please send me your Cureus email. Thank you
07 August 2023 1,870 3 View
Uso da ferramenta - The Impact of Events Scale-Revised (IES-R)
26 February 2023 6,223 0 View
In the Raman technique, can the laser of the equipment itself change the surface structure of an organic polymer that was previously irradiated with gold ions? If the Raman laser itself is...
25 February 2023 1,758 1 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have been performing short phagocytosis experiments using 96-round bottom, tissue culture treated, well plates (Falcon, 353077). I culture the cells with the phagocytic cargo for 2-4hrs, wash...
04 August 2024 5,228 4 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
In my study, I intend to infect PBMCs with SARS-CoV-2. After that, I will analyze NK cells by flow cytometry to see if their phenotype changes or if they show degranulation. After the infection, I...
01 August 2024 4,403 4 View
I have used PE and PE-Dazzle 594 fluorochromes and have managed to get NK cells to properly show GranzymeB expression after 4 hr PMA/ionomycin stimulaton, but for some reason my CD8 cells in the...
01 August 2024 7,677 2 View