Hi there,

If you demonstrate that the purified protein is responsible for a specific biological activity in vitro, if you manage to cocrystallize it with substrate (or analogs) and that the 3D structure resembles the published structures of homologous protein, it would be more than convincing.

Thanks Dominique. What if the protein is showing only 60-70 percent activity. I mean the protein used for crystallization still contains some inactive protein?

Hi again,

If your protein prep is a mixture of different populations it will make the crystallization step difficult. How do you know your prep is partially active only? Is it homogenous when passed on gel filtration?

Hi. I am thinking of doing activity assay for my refolded protein. I found in many papers that particular protein is showing 60% or 70% activity after refolding. If I get, suppose 60% activity, can I go for crystallization.