If you synthesized silver nanoparticles from silver ions in the presence of a plant reducing agent. From 1 g of silver ions in the precursor, you should get 1 g of silver nanoparticle. The obtained silver nanoparticles should be well cleaned and proved by the IR spectrum that there are no organic substances on their surface, i.e. there are no functional groups. Then you weigh and get 0.5 g. Therefore, you received 50% of the theoretical yield of the reaction.

Prof Yuri, what will be the stability of nanoparticles if you remove all surface groups? it will aggregate as I feel. We generally, centrifuge the particles at 13000-15000 rpm and redisperse in water and repeat it several times to get the silver or gold nanoparticles without unreacted materials. Then simple spectroscopic techniques can be applied to calculate the concentration if you know the molar absorptivity.

Mr. Yuri, can you share any research paper regarding this method as reference?

Yuri Mirgorod