Simulated digestion breaks protein into peptides, so using Bradford method will be an accurate method or not? Please suggest me. Also suggest any other chemical methods with references.
Dear Md Faisal,
The Bradford method for protein quantification will not be ideal for peptide quantification. I would recommend the Pierce™ Quantitative Colorimetric Peptide Assay for measuring peptides in solution.
(https://www.thermofisher.com/order/catalog/product/23275)
Good luck.
Best,
Peter
***
The Thermo Scientific™ Pierce™ Quantitative Colorimetric Peptide Assay is an easy-to-use colorimetric microplate assay designed specifically to improve the sensitivity and reproducibility of quantitation of peptide mixtures.
Sensitive—accurately detect as little as 25 µg/mL of peptide mixture
Robust—assay performance rigorously tested using peptide digest mixtures
Robust peptide digest standard—kit includes a validated peptide digest standard for improved reproducibility of quantitation
Compatible—works with many reagents, including mass spectrometry sample preparation reagents and TMT™-labeled peptides
Convenient—easy mix-and read-format and stable colorimetric signal that may be read in as little as 15 minutes.
Hi,
You can use Macro Kjeldahl method for Total protein but digestion breaks protein detected by electrophoresis.
Dear Dr Peter Allen,
Thanks a lot for your guideline and link.
Regards
Md Faisal
Dear Dr Alaa Kareem Niamah,
Thanks a lot for your guideline and link.
Regards
Md Faisal
as i think you can use Macro Kjeldahl method for Total protein but digestion breaks protein detected by electrophoresis.
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