Is it possible(or logical) to notice an elevated autophagy using LC3-II based immunoblotting, but no difference by using GFP-LC3 based imaging study?

For example, I treat cells with "X" - performed immunoblotting for LC3-II - found elevated autophagy, then did the same treatment in GFP-LC3 transfected cells followed by confocal imaging - found...

06 April 2020 4,332 4 View

Any Suggestions to rectify negative intercept in HPLC?

Drug is Sodium citrate, diluting drug in de-ionized water. Using a C18 column. the concentration range of 1000-3000microgram to lower range of 25-300microgram. Thanks!

19 September 2019 7,315 3 View

Can any one help to get complete bandwidth from 2 to 12 GHz which was mentioned in this paper using HFSS?

can any one help to get complete from 2 to 12 GHz bandwidth which was mentioned in this paper using HFSS... I got 5 to 12 GHz bandwidth Paper Title: Small Square Monopole Antenna With Inverted...

17 September 2019 3,508 3 View

What would be the mobile phase for quantifying sodium citrate using HPLC method?

I have to determine concentration of sodium citrate using HPLC method. Previous literature's used potassium phosphate buffer of pH 2.5 as mobile phase (isocratic). As there is risk of clogging the...

31 July 2019 1,101 5 View

Do we have a specific model and strategy for industry-university collaboration on autonomous vehicle products and services?

The challenge faced by the autonomous vehicle industry is to keep pace with the exploration of autonomous vehicles. The industrial research centers are limited and prevent them from sharing their...

08 June 2019 5,916 3 View

Why wavelength 546nm (UV) is chosen to check incompatibility of 2 drug solutions?

Looking for Turbidity of a solution defined as an absorbance value >0.015OD, which is measured using UV equipment. Wavelength used in most of the incompatibility papers is 546nm, irrespective...

30 May 2019 5,737 3 View

What would be a good brief food frequency questionnaire for use in parents of elementary school aged children?

I understand from the literature that child reports are more accurate than parent reports for school children, but wanted to specifically look at a brief ffq of parents for the same .

04 April 2019 8,643 1 View

How to validate the researcher as an instrument in qualitative research?

I am post doctoral student conducting a qualitative research. One of my thesis correction requirement was for me to state how I validate the researcher as an instrument since the researcher is...

24 December 2018 787 3 View

Example survey question for measuring efficiency in textile production unit?

creating a survey questionnaire for my research project.

02 September 2018 3,352 1 View

What is the detailed procedure for brush coating of commercial and synthesized Graphene oxide on Gas Diffusion Layer for ORR in fuel cells?

I have tried several times brush coated the commercial graphene oxide (CGO) on Gas diffusion layer (GDL) but every time the coating forms thin stripes (like zeebra stripes) and not at all...

28 February 2018 9,639 3 View

Polymer composites suddenly solidifies?

I am making polymer composite by solvent mixing method. When I am trying to add filler solution in polymer solution it quickly get solidified. How to make them then uinform? It doesn't give time...

03 March 2021 7,540 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

How to polymerize TEOS?

I want to polymerize TEOS on a surface. I know that NH4OH is a preferred catalyst and an alcoholic solvent should be used .But I can not find certain amounts of each material to use. Does any one...

02 March 2021 1,404 6 View

Why Austenite grains in steel show much more twinning than ferrite grains?

It is oftentimes said to be a distinctive metallographic trait that differentiates a fully austenitic and a fully felly ferritic steel without etching that might distinguish between two. Why...

02 March 2021 728 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

How can extract ciprofloxacin hydrochloride disolve in water er to organic solvent such as dichloromethan or any other solvent?

I need a procedure to extract the ciprofloxacin hydrochloride from aqueous solution by using dichloromethane in detail,. I thank you very much.

01 March 2021 4,306 1 View

How to separate water from glycerol solvent in a solvent recovery vacuum distillation column?

I am currently doing my third year design project. I have been assigned to design a glycerol recovery column in vinyl acetate monomer production. The process unit before the recovery column is an...

01 March 2021 9,525 4 View

How to make fractions of chloroform extract of plant, using seperating funnel?

28 February 2021 8,466 2 View

Why am I not able to use all VEP programs for the in silico analysis of avpr1b SNP's?

Good day researchers I am busy analysing the predicted effects of certain SNP's in AVPR1b. When using the VEP prediction programs (SIFT, Polyphen, FATHMM, LRT, Provean, Mutation Assessor, and...

28 February 2021 5,197 1 View