I plan to treat SCID NCr mice with a drug candidate formulated in 5%DMSO, 5% Tween80 and 40% PEG300. The formulation will be delivered by IV.
We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...
17 July 2024 7,767 3 View
Hi expert, My pushover curve doesnt showing any yeilding point (SAP2000) produce a straight curve, this happens on the X-axis, and my building is shaped like a stadium, what makes it wrong?
11 June 2024 6,225 3 View
These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...
06 June 2024 2,289 3 View
I am using PerioPpaer from Oraflow, to collect GCF, 8-12 PerioPapers from each subject and a pooled sample in PBS elution buffer. For the elution, I am vortexing for 1 min, then slow...
03 June 2024 9,767 0 View
I performed single-point energy optimized using Gaussian software, followed by mapping the electrostatic potential surface around that. There is a clear dipole for that molecule but I want to...
24 May 2024 8,549 1 View
Optimal power flow, small hydro
20 May 2024 7,590 0 View
I have a video of Brownian motion of microbes under a microscope. From this video, I need to calculate the angular velocity of a particular prominent cell. From my understanding, what I know is...
18 May 2024 4,289 3 View
I need to select 3 best results from the list of the 17 antiviral drugs. How do I select that?
25 April 2024 5,386 1 View
explain its morphology, taxonomy and habitat distributiion
03 March 2024 1,293 1 View
I have checked auto activation for our bait strain by 200ng aureobasidin A but still healthy colonies we got.
21 February 2024 8,061 0 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am experimenting with cancer and non-cancer cells in a 12-well plate for 4 days with a seeding density 1*10^4/well, however, I noticed that the control group growth rate slows down on D3. Should...
07 August 2024 2,283 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....
04 August 2024 762 1 View
I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...
03 August 2024 4,725 2 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View
Hello everyone, I need to dissolve stigmasterol for an animal experiment, but it hasn't dissolved in either DMSO or vegetable oil. Can anyone recommend the best solvent for stigmasterol?
01 August 2024 7,504 2 View