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I am getting the following error in DNAsubway purple line on Cyverse.

For the metadata file with the name Albopictus.csv:

Errors:

• file [Aalbo_R1.fastq.gz] is not well named
• file [Aalbo_R2.fastq.gz] is not well named
• no data files found for valid sample ID [Albo]

Warnings:

• categorical metadata B must include at least 2 unique values
• categorical metadata body-site must include at least 2 unique values
• categorical metadata year must include at least 2 unique values
• categorical metadata month must include at least 2 unique values
• categorical metadata day must include at least 2 unique values
• categorical metadata subject must include at least 2 unique values

Header columns:

• #SampleID
• BarcodeSequence
• LinkerPrimerSequence
• B
• body-site
• year
• month
• day
• subject
• Description

Error and warning message is indicating what the errors are. Fix them before you proceed.

Follow all the messages that are warned you because it is indicating what is your errors.

Muhammad Fahim you are missing the "forward-absolute-filepath" and "reverse-absolute-filepath" as headers columns. Without this you won´t be able to read the sequences.