Do we need to write protocol for a second study of a systematic review that has been registered and published?

I have conducted and published a systematic review and meta-analysis research with the topic related to public health and health pomotion (protocol was registed in PROSPERO). Now we want to...

03 March 2021 8,920 3 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Where can the fault diagnosis data set of lithium battery be found?

Most of the data sets on the network only contain good information about the voltage and internal resistance of lithium batteries. The data of faulty battery is missing. Who can help me, I will be...

21 February 2021 8,659 1 View

Where can I find these two papers?

14 January 2021 1,572 3 View

How can I digest the formaldehyde (1%) crosslinked DNA-protein with restriction enzyme (DpnII) better?

I am working on yeast samples for 4C-seq study , but the formaldehyde fixed samples can not be digested with restriction enzyme (DpnII). Please help me out. Thanks.

28 August 2020 6,483 3 View

Could anyone help me to determine the structure of this molecule?

Dear all, I need to determine the structure of the following molecule, see the addached file, could anyone help me? Thanks very much!!!

22 June 2020 9,128 1 View

Recommendations for constitutive expression plasmid in K12-BW25113 E.coli?

Dear researchers, I would like to ask for the recommendation for plasmid (kan or chloramphenicol resistance marker) which could constitutively express the E.coli protein (inner membrane protein)...

11 November 2019 7,968 1 View

Could tamoxifen citrate be solubilized in corn oil?

Hi, I am going to inject tamoxifen in mice at 2 mg/mouse/day, and I plan to prepare 20 mg/mL solution in corn oil as Jackson lab suggested. But I noticed that they use tamoxifen free base, but we...

17 September 2019 8,673 1 View

How do we make multi Fatsa files?

Hi, previously I had used MUMer to run but the input are only able to upload 1 Fasta file. When I test run a reference and the sample it work great but when I combine my sample 1 and sample 2 data...

25 July 2019 3,681 1 View

Why lower molecular weight protein bands disappeared in Tricine SDS PAGE?

Hi, I run this gel using 3 different layers: -the upper layer: 4%T 3%C stacking gel -the middle layer: 10%T 3%C spacer gel (1cm) -the lower layer: 16%T 6%C separating gel -voltage: 60V until the...

17 June 2019 9,711 1 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View

Want to ask about equivalence ratio?

09 February 2021 5,273 2 View

In Michaelis-Menten Kinetics, why K(subscript)On have units (mol^-1)(time^-1)?

where K(subscript)On is the rate of Enzyme (E) + Substrate (S) going to Enzyme-Substrate complex (ES). I think I understand why K(subscript)Off and K(subscript)catalysis have units of time^-1....

11 January 2021 649 8 View

Do chemical and physical properties influence on the size of a polymer particle?

I would like to know if the properties of a polymer impact on the size of the particle it forms. I'm working with different polyssacharides (alginic acid from different seaweed), which are...

29 December 2020 7,333 6 View

How to make solution with molar ratio 1:40?

Currently i'm working to develop encapsulation using ferritin, based on many journals they always wrote the molar ratio between drugs and ferritin, for example 1:40, how to know the molarity that...

22 December 2020 6,582 2 View

Having issues understanding the particles/mL to molarity conversion, I've seen many different way but non of them seem to match one an the other?

I have 68nm spherical Au NPs, which gave me a concentration of 1.22 x 10^9 particles/mL. How can I calculate the molarity without the need for TEM?

22 November 2020 8,850 3 View

What solvent can I use to prepare a higher concentration of 5-Bromouracil?

I am standardizing a UHPLC method for separation of thioguanine phosphate metabolites. For this, I want to use 5-Bromouracil (5-BU) as an internal standard, however I am unable to prepare solution...

15 November 2020 4,982 3 View

How storage capacity for clathrate hydrate is calculated?

Storage capacity of hydrate solely depends on molar volume of empty hydrate lattice because rest of the terms get canceled (even moles of gas consumed). However, if we take temperature constant,...

16 October 2020 720 2 View

Please suggest that the way of obtaining the concentration (in mol) from the gas mixture (N2=98%, Ethylene=1% and Butadiene=1%) is correct or not?

I have a gas mixture (N2=98%, Ethylene=1% and Butadiene=1%). I want to obtained the molar concentration of each components for 1mL volume of the gas mixture. For that purpose, I have used the...

29 September 2020 6,233 1 View

How can I make a HgSO4 1M solution?

Hi everyone I want to make a HgSO4 1 Molar solution for deposition (electroplating) in my electrode, but HgSO4 is not soluble in water! What can I do !? Because My other solutions are all in water.

14 September 2020 3,461 3 View