I have a duplex DNA at 188bp which have been reconstituted to 100uM. I know to get the MW is 188bp * 660 g/mol = 124080. From here I'm not quite sure how to work out the copy number. Can someone help me please?
This graph shows that the rate of increase of uremia patients exceeds the global population growth. Does anyone know where this picture came from?
13 June 2024 2,635 0 View
Alkali Blue 6B and Acid Blue 119 are the same compound in the measurement of Acidity in edible oil and fat? If not, color range by pH for those compounds is different? e.g. pH 9.4 (blue-violet)...
04 February 2024 5,692 0 View
My research is mainly about anthropomorphism\attachment anxiety\loneliness\mentalizing, I want to know the latest research about my interest.
14 November 2023 6,073 2 View
Hi, I have set of experimental data that I have collected from fatigue tests and I would like to ask how do I curve fit the data with the BS 7608 standard equation on excel? I have tried various...
05 November 2023 477 1 View
Hi, I've been working on DIG-labeling northern blotting for a while, but recently i get some troubles regard to the background. As the picture below, i keep having these non-specific bands in my...
04 November 2023 4,672 0 View
Hello every bio-field expert, professor, researcher: Our experiment design aims to induce the immune system, to affect the tumor cell progress. In the previous study, we found a connection...
19 October 2023 7,899 2 View
Let's say I have the values of my material properties (e.g. UTS, Yield Strength, Young's modulus) after conducting a tensile test, and then I would proceed to conduct a fatigue test to investigate...
21 August 2023 608 2 View
When there is no magnetic field in the simulation, emittances given by the postprocessing and my own code are same. But when there is magnetc field in the simulation, no matter how small of the...
07 June 2023 1,514 2 View
If you are animation or film related experts, please let me to do this survey. I am sure it will be also benefit to you in both academic and practical projects. Survey link...
22 May 2023 180 0 View
After learning and understanding, we know that GRACE Level 2 RL06 data needs further post-processing before it can be used. Including the replacement of C20 items, C21, S21 items, different...
10 May 2023 6,728 1 View
Hello, Can anyone provide me with the absorption coefficient of methane gas at 7.7 um? Any reference?
06 August 2024 980 5 View
How to calculate the molar proportions of the oxides from the XRF analyses?
02 August 2024 2,565 2 View
NA
11 July 2024 8,969 1 View
I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...
02 July 2024 612 6 View
I'm currently working on preparing a 10 mL liposome solution containing HSPC, mPEG2000-DSPE, and cholesterol at a molar ratio of 55/40/5. can anybody advise on the best way to calculate the weight...
26 June 2024 1,135 0 View
The percentage molar concentration of lactic acid and CTAB
20 June 2024 3,225 0 View
Good evening, I´m establishing a protocol for detecting ROS using DHE staining in Flow Cytometry using TK6 cells. They look visibly pink after staining them with 10 micro Molar DHE. Is that...
19 June 2024 7,339 0 View
I want to prepare a photocatalytic material Bi3TiNbO9 nanosheets and the authors mentioned NaCl-KCl (8 g), a molar ratio of 1:1 and an eutectic temperature of 657 °C as a one component. So, kindly...
04 May 2024 9,282 1 View
I am trying to optimise the molar ratio of 2 analyte (For example A and B) during a reaction. For this purpose I want to optimise there molar ratio. For example A:B = 1:2, 1:3, 1:4, etc. Which...
28 April 2024 5,775 2 View
Hi all, I want to have 301 copies/ul for my downstream experiments. The sample I am using is a purified plasmid, of which I know the concentration (ng/ul), volume (ul), and molarity(g/mol). How...
20 April 2024 2,468 4 View