I used size exclusion column superdex 75 increase to purify gold nanoclusters, but some of them stuck on the column, how could I wash out them? Will 0.1 M HCl work?
first try more polar, slightly basic aqueous buffer as the mobile phase, and if that is not effective, then consider increasing the ionic strength before resorting to the acidic 0.1 M HCl wash. The key is to find conditions that can disrupt the non-size-based interactions without compromising the integrity of the gold nanoclusters.
The integrity of the superdex gel is the one at risk. Some of the clusters enter some of the pores and got stuck there. In my experience, it is difficult to remove them without harming the gel. Something that disrupts the cluster could work, a high concentration of cysteine containing molecule might work.
You can try rinsing with 5-10 mmol/L dithiothreitol (DTT) solution or 0.1% trifluoroacetic acid (TFA)/acetonitrile.
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