I used Olympus CellSens software to take staked pictures (.vsi). Open the file using ImageJ FIJI with Bioformat plugin but picture appeared more. purple so how can I see the original colors of HE stained as I saw it under microscope.
To visualize an H&E (Hematoxylin and Eosin) stained slide on ImageJ with its original colors, you can follow these steps:
1. Open ImageJ:
- If you haven't already, download and install ImageJ from the official website (https://imagej.nih.gov/ij/download.html).
2. Load the H&E stained image:
- Go to "File" > "Open" and select the H&E stained image you want to visualize with its original colors.
3. Split Channels:
- Once the image is open, go to "Image" > "Color" > "Split Channels."
- This action will split the image into its individual channels: the hematoxylin (H) channel and the eosin (E) channel.
4. Merge Channels with Original Colors:
- Go to "Image" > "Color" > "Merge Channels..."
- In the "Merge Channels" dialog, select the "Red" channel and choose the H channel from the drop-down menu.
- Select the "Green" channel and choose the E channel from the drop-down menu.
- Leave the "Blue" channel as "None" (unless you have a third channel representing another stain).
- Make sure the "Create Composite" checkbox is checked.
- Click "OK."
Now, the H&E stained image should be visualized with its original colors as closely as possible, with the hematoxylin (nuclei) stained regions appearing in shades of blue and the eosin (cytoplasm) stained regions appearing in shades of pink/red.
Please note that while this method will approximate the original colors, the exact appearance may vary depending on the image and staining conditions. Additionally, this method assumes that the H&E stained image is a standard RGB image, and it may not work for images with other color spaces or special color mapping.
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