Hello! How to view the UV-spectrum on the shimadzu SPD 20A device? My HPLC does not have a PDA detector. We have a HPLC in the laboratory with PDA detector and on this chromatograph it is very easy to see the UV spectrum. I cannot find the tab where the UV spectrum is displayed. This is about offline mode.
Thanks in advance
What detector do you have on your LC system? You question is confusing because the SPD-20A is a PDA detector, but you then state that your HPLC doesn't have a PDA detector.
Make sure that you have spectral analysis turned on during your runs. If you don't collect the data, you can't analyze it later.
What software are you using? does it support that feature of the SPD-20A?
Jack Silver , hanks for your response. I am guided by our second HPLC, which has an array detector. And I can look at the range from 200 to 800 nm at any point on the chromatogram. The new chromatograph has a simple UV detector. And on it I cannot visualize such a range. This is my question. How to visualize the range from 200 to 800 nm on a simple (non-matrix) UV detector. Lab solutions program.
If if is a "simple UV detector" it probably only monitors one or two wavelengths. What model model is the new detector? Is it a Shimadzu?
Jack Silver, yes it's shimazu. SPD-20A. It turns out that only on the matrix detector you can see the range from 200 to 800 nm?
To see the UV spectra on a Shimadzu system you have to have a PDA detector. In the case of just a UV detector the software installed does not support even showing the UV spectra window. So, if you do have a PDA version of LabSolutions you can open that window by going to Postrun Analysis and selecting "Window" from the top menu (right before "Help") and scrolling down to "UV Library Editor"
Victor Alexandrovich Nikolaev thank you very much. This information was very helpful.
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